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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
High frequency Agrobacterium-mediated transformation and plant regeneration via direct shoot formation from leaf explants in Beta vulgaris and Beta maritima
| Title: | High frequency Agrobacterium-mediated transformation and plant regeneration via direct shoot formation from leaf explants in Beta vulgaris and Beta maritima |
| Authors: | Hisano, H. Browse this author | | Kimoto, Y. Browse this author | | Hayakawa, H. Browse this author | | Takeichi, J. Browse this author | | Domae, T. Browse this author | | Hashimoto, R. Browse this author | | Abe, J. Browse this author →KAKEN DB | | Asano, S. Browse this author →KAKEN DB | | Kanazawa, A. Browse this author →KAKEN DB | | Shimamoto, Y. Browse this author |
| Keywords: | Agrobacterium tumefaciens | | Beta vulgaris | | Regeneration | | Shoot-base tissue | | Transformation |
| Issue Date: | Jul-2004 |
| Publisher: | Springer Berlin / Heidelberg |
| Journal Title: | Plant Cell Reports |
| Volume: | 22 |
| Issue: | 12 |
| Start Page: | 910 |
| End Page: | 918 |
| Publisher DOI: | 10.1007/s00299-004-0773-3 |
| PMID: | 15042407 |
| Abstract: | Communicated by H. Uchimiya Abstract We have developed a new procedure for Agrobacterium-mediated transformation of plants in the genus Beta using shoot-base as the material for Agrobacterium infection. The frequency of regeneration from shoot bases was analyzed in seven accessions of sugarbeet (Beta vulgaris) and two accessions of B. maritima to select materials suitable for obtaining transformed plants. The frequency of transformation of the chosen accessions using Agrobacterium strain LBA4404 and selection on 150-mg/l kanamycin was found to be higher than that in previously published methods. Genomic DNA analysis and -glucuronidase reporter assays showed that the transgene was inherited and expressed in subsequent generations. In our method, shoot bases are prepared by a simple procedure, and transformation does not involve the callus phase, thus minimizing the occurrence of somaclonal variations. |
| Rights: | The original publication is available at www.springerlink.com |
| Relation: | http://www.springerlink.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/16870 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 金澤 章
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