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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
Lipid raft disruption prevents apoptosis induced by 2-chloro-2'-deoxyadenosine (Cladribine) in leukemia cell lines.
| Title: | Lipid raft disruption prevents apoptosis induced by 2-chloro-2'-deoxyadenosine (Cladribine) in leukemia cell lines. |
| Authors: | Takahashi, Eriko Browse this author | | Inanami, Osamu Browse this author →KAKEN DB | | Ohta, Toshio Browse this author →KAKEN DB | | Matsuda, Akira Browse this author →KAKEN DB | | Kuwabara, Mikinori Browse this author |
| Keywords: | Apoptosis | | Leukemia | | 2-Chloro-2'-deoxyadenosine | | Lipid rafts | | Calcium | | L-type Ca2+ channel |
| Issue Date: | Dec-2006 |
| Publisher: | Elsevier Ltd. |
| Journal Title: | Leukemia Research |
| Volume: | 30 |
| Issue: | 12 |
| Start Page: | 1555 |
| End Page: | 1561 |
| Publisher DOI: | 10.1016/j.leukres.2006.04.008 |
| PMID: | 16730061 |
| Abstract: | To clarify the role of lipid rafts in 2-chloro-2′-deoxyadenosine (2CdA; Cladribine)-induced apoptosis, the effects of disruption of lipid rafts by methyl-β-cyclodextrin (MβCD) and filipin on 2CdA-induced apoptosis were investigated in four human acute lymphoblastic leukemia (ALL) cell lines comprised of T cells (MOLT-4, Jurkat) and B cells (NALM, BALL-1). The disruption of lipid rafts significantly inhibited 2CdA-induced apoptosis, indicating the crucial role of lipid rafts in the induction of apoptosis in leukemia cells. These reagents significantly inhibited 2CdA-induced elevation of the intracellular calcium concentration ([Ca2+]i) in MOLT-4 cells, and 2CdA-induced apoptosis was partly inhibited by the Ca2+ chelators BAPTA-AM and EGTA, and the L-type Ca2+ channel blocker nifedipine. On the other hand, they had no effects on the cellular uptake of 2CdA. These results indicated that lipid rafts partly contributed to 2CdA-induced apoptosis by regulating Ca2+ influx via the plasma membrane. |
| Relation: | http://www.sciencedirect.com/science/journal/01452126 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/16953 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 桑原幹典
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