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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >
Parentage assignment in hatchery population of brown sole Pleuronectes herzensteini by microsatellite DNA markers
| Title: | Parentage assignment in hatchery population of brown sole Pleuronectes herzensteini by microsatellite DNA markers |
| Authors: | Kim, Sang-Gyu Browse this author | | Morishima, Kagayaki Browse this author | | Satoh, Nobukazu Browse this author | | Fujioka, Takashi Browse this author | | Saito, Setsuo Browse this author | | Arai, Katsutoshi Browse this author →KAKEN DB |
| Keywords: | effective population size | | genetic diversity | | hatchery population | | heterozygosity | | microsatellite DNA | | parentage assignment | | Pleuronectes herzensteini |
| Issue Date: | Oct-2007 |
| Publisher: | Japanese Society of Fisheries Science |
| Journal Title: | Fisheries Science |
| Volume: | 73 |
| Issue: | 5 |
| Start Page: | 1087 |
| End Page: | 1093 |
| Publisher DOI: | 10.1111/j.1444-2906.2007.01440.x |
| Abstract: | Five loci (Phz2, Phz6, Phz7, Phz12, and Phz14) of microsatellite DNA markers developed in a previous study for parentage assignment in the hatchery population generated by mating among 61 broodstock fish (35 females and 26 males) in a spawning tank, were selected. After natural spawning in the same tank, larvae collected at three different times were categorized into early phase (EP), middle phase (MP), and late phase (LP) groups. In the parental broodstock, the mean number of alleles per locus was 21.8 and expected heterozygosity (HE) was 0.813. In the progeny, the mean number of alleles per locus decreased to 11.6 (EP), 14.4 (MP), and 6.4 (LP) and HE to 0.796 (EP), 0.833 (MP), and 0.681 (LP). Parental assignment determined eight dams and six sires as major parents for the EP group. In the MP group, 13 dams and ten sires genetically contributed to spawning, but only three dams and two sires were involved in LP group progeny. In the hatchery population produced from a limited number of parental fish such as the LP group, genetic variability was apparently decreased. |
| Rights: | © 2007 公益社団法人日本水産学会 | | © 2007 The Japanese Society of Fisheries Science |
| Type: | article |
| URI: | http://hdl.handle.net/2115/35582 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 荒井 克俊
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