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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
北海道大学水産科学研究彙報 = Bulletin of Fisheries Sciences, Hokkaido University >
第59巻 第2号 >
加熱損傷ウェルシュ菌の検出培地の比較と改良
| Title: | 加熱損傷ウェルシュ菌の検出培地の比較と改良 |
| Other Titles: | Comparison and Improvement of Detection Media for Sub-lethal Injured Clostridium perfringens |
| Authors: | 清水, 茂雅1 Browse this author | | 堀口, 明日香2 Browse this author | | 山崎, 浩司3 Browse this author →KAKEN DB | | 川合, 祐史4 Browse this author →KAKEN DB |
| Authors(alt): | Shimizu, Shigemasa1 | | Horiguchi, Asuka2 | | Yamazaki, Koji3 | | Kawai, Yuji4 |
| Keywords: | Clostridium perfringens | | Injured cell | | Recovery | | Sodium pyruvate |
| Issue Date: | 3-Aug-2009 |
| Publisher: | 北海道大学大学院水産科学研究院 |
| Journal Title: | 北海道大学水産科学研究彙報 |
| Journal Title(alt): | Bulletin of Fisheries Sciences, Hokkaido University |
| Volume: | 59 |
| Issue: | 2 |
| Start Page: | 37 |
| End Page: | 42 |
| Abstract: | Recovery of heat-injured vegetative cells and spores of Clostridium perfringens was evaluated on selective media, CW Egg-yolk agar with kanamycin (ECW+A), Tryptose sulfite cycloserine agar(TSC+A)and modified Handford agar (mHFA). As a result of heat treatment at 54℃ for 15 min, viable counts of C. perfringens vegetative cells on a selective medium (ECW+A)was significantly less than those on non-selective media. This means C. perfringens vegetative cells should be in an injured state. Comparing of the three selective media, TSC+A was the best growth and recovery of heat-injured C. perfringens vegetative cells. Recovery on ECW+A was 1 log CFU/ml less than that on TSC+A. Supplementation of TSC+A with sodium pyruvate (0.1-0.5%) further enhanced recovery and detection of heat-injured C. perfringens vegetative cells, and its efficacy was the highest on TSC+A supplemented with 0.3% sodium pyruvate. Supplementation of TSC+A with sodium pyruvate did not affect recovery of heat-injured (95℃, 2 or 60 min) C. perfringens spores. These findings suggest that TSC+A is the most favorable medium for enumeration of C. perfringens and supplementation with sodium pyruvate improves recovery and detection of heat-injured C. perfringens. |
| Type: | bulletin (article) |
| URI: | http://hdl.handle.net/2115/39007 |
| Appears in Collections: | 北海道大学水産科学研究彙報 = Bulletin of Fisheries Sciences, Hokkaido University > 第59巻 第2号
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