| Title: | pfaB products determine the molecular species produced in bacterial polyunsaturated fatty acid biosynthesis |
| Authors: | Orikasa, Yoshitake Browse this author |
| Tanaka, Mika Browse this author |
| Sugihara, Shinji Browse this author |
| Hori, Ryuji Browse this author |
| Nishida, Takanori Browse this author |
| Ueno, Akio Browse this author |
| Morita, Naoki Browse this author |
| Yano, Yutaka Browse this author |
| Yamamoto, Kouhei Browse this author |
| Shibahara, Akira Browse this author |
| Hayashi, Hidenori Browse this author |
| Yamada, Yohko Browse this author |
| Yamada, Akiko Browse this author |
| Yu, Reiko Browse this author |
| Watanabe, Kazuo Browse this author |
| Okuyama, Hidetoshi Browse this author →KAKEN DB |
| Keywords: | docosahexaenoic acid |
| eicosapentaenoic acid |
| Moritella marina strain MP-1 |
| polyunsaturated fatty acid |
| pfaB gene |
| Issue Date: | Jun-2009 |
| Publisher: | Blackwell Publishing |
| Journal Title: | FEMS Microbiology Letters |
| Volume: | 295 |
| Issue: | 2 |
| Start Page: | 170 |
| End Page: | 176 |
| Publisher DOI: | 10.1111/j.1574-6968.2009.01582.x |
| PMID: | 19453514 |
| Abstract: | When pDHA4, a vector carrying all five pfaA-pfaE genes responsible for docosahexaenoic acid (DHA; 22:6) biosynthesis in Moritella marina MP-1, was coexpressed in Escherichia coli with the individual pfaA-pfaD genes for eicosapentaenoic acid (EPA; 20:5) biosynthesis from Shewanella pneumatophori SCRC-2738, both polyunsaturated fatty acids were synthesized only in the recombinant carrying pfaB for EPA synthesis. Escherichia coli coexpressing a deleted construct comprising pfaA, pfaC, pfaD and pfaE for EPA and pfaB for DHA produced EPA and DHA. Both EPA and DHA were detected in bacteria that inherently contained pfa genes for DHA. These results suggest that PfaB is the key enzyme determining the final product in EPA or DHA biosynthesis. |
| Rights: | The definitive version is available at www.blackwell-synergy.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/43086 |
| Appears in Collections: | 環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
