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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Dental Medicine / Faculty of Dental Medicine >
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The diacylated lipopeptide FSL-1 induces TLR2-mediated Th2 responses
| Title: | The diacylated lipopeptide FSL-1 induces TLR2-mediated Th2 responses |
| Authors: | Kiura, Kazuto Browse this author | | Kataoka, Hideo Browse this author | | Yasuda, Motoaki Browse this author | | Inoue, Nobuo Browse this author | | Shibata, Ken-ichiro Browse this author →KAKEN DB |
| Keywords: | adjuvant | | Th2 response | | Toll-like receptor 2 | | lipopeptide |
| Issue Date: | Oct-2006 |
| Publisher: | 55 |
| Journal Title: | FEMS immunology and medical microbiology |
| Volume: | 48（１） |
| Start Page: | 44 |
| End Page: | 55 |
| Publisher DOI: | 10.1111/j.1574-695X.2006.00119.x |
| PMID: | 16965351 |
| Abstract: | The diacylated lipopeptide FSL-1 enhanced the generation of IgG antibodies in TLR2+/+ mice, but not in TLR2-/- mice, when administered together with hen egg lysozyme as an antigen. Escherichia coli lipopolysaccharide enhanced the generation of antigen-specific antibodies in both TLR2-/- and TLR2+/+ mice. In TLR2+/+ mice, the enhancement level by FSL-1 was similar to that by lipopolysaccharide. Analysis of the IgG antibodies subclass demonstrated that level of Th2-type IgG1 antibodies was higher than that of Th1-type IgG2a antibodies. Both FSL-1 and lipopolysaccharide induced production of IL-10 and IL-6 by splenocytes from TLR2+/+ mice. Lipopolysaccharide also induced production of these cytokines by splenocytes from TLR2-/- mice, but FSL-1 did not. Neither FSL-1 nor lipopolysaccharide induced IL-12p70 production by splenocytes from both types of mice. FSL-1 upregulated B7.2 expression in B220+ cells from TLR2+/+ mice but not those from TLR2-/- mice, whereas lipopolysaccharide upregulated B7.2 expression in B220+ cells from both types of mice. FSL-1 and, to a lesser extent, lipopolysaccharide activated mitogen-activated protein kinases in splenocytes. FSL-1 and, to a lesser extent,lipopolysaccharide induced the expression of c-Fos, which is known to be involved in Th2-type responses, in splenocytes. Thus, this study demonstrated that FSL-1 possessed TLR2-mediated Th2-type responses in vivo. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/43974 |
| Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 柴田 健一郎
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