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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
Induction of neurite outgrowth by α-phenyl-N-tert-butylnitrone through nitric oxide release and Ras-ERK pathway in PC12 cells
| Title: | Induction of neurite outgrowth by α-phenyl-N-tert-butylnitrone through nitric oxide release and Ras-ERK pathway in PC12 cells |
| Authors: | Yasui, Hironobu Browse this author →KAKEN DB | | Ito, Nozomi Browse this author | | Yamamori, Tohru Browse this author →KAKEN DB | | Nakamura, Hideo Browse this author →KAKEN DB | | Okano, Jun Browse this author | | Asanuma, Taketoshi Browse this author | | Nakajima, Takayuki Browse this author | | Kuwabara, Mikinori Browse this author | | Inanami, Osamu Browse this author →KAKEN DB |
| Keywords: | Antioxidant | | neurogenesis | | nitric oxide |
| Issue Date: | Jun-2010 |
| Publisher: | Informa Healthcare |
| Journal Title: | Free Radical Research |
| Volume: | 44 |
| Issue: | 6 |
| Start Page: | 645 |
| End Page: | 654 |
| Publisher DOI: | 10.3109/10715761003692537 |
| PMID: | 20370559 |
| Abstract: | It has previously been suggested that the spin trap agent α-phenyl-N-tert-butylnitrone (PBN) induces neurite outgrowth through activation of the Ras-ERK pathway in PC12 cells. However, the chemical properties of PBN contributing to its biological function and the detailed mechanism for the activation of Ras by PBN remain unknown. This study demonstrates that the hydrophobic structure of PBN is related to the activation of Ras, by comparing with hydrophilic analogues of PBN. [14C]-labelled PBN was found to localize in the lipid fraction and activate Ras indirectly. On the other hand, neurite outgrowth by PBN was inhibited by a nitric oxide (NO) scavenger. Moreover, the neurite outgrowth induced by PBN and the NO donor NOR4 was inhibited by the dominant negative Ras or MAPK/ERK inhibitor. Taken together, these results suggest that PBN is incorporated into the plasma membrane and induces neurite outgrowth in PC12 cells by activating the Ras-ERK pathway through NO release |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/46751 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 稲波 修
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