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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Medicine / Faculty of Medicine >
Peer-reviewed Journal Articles, etc >
Localization of diacylglycerol lipase-alpha around postsynaptic spine suggests close proximity between production site of an endocannabinoid, 2-arachidonoyl-glycerol, and presynaptic cannabinoid CB1 receptor.
| Title: | Localization of diacylglycerol lipase-alpha around postsynaptic spine suggests close proximity between production site of an endocannabinoid, 2-arachidonoyl-glycerol, and presynaptic cannabinoid CB1 receptor. |
| Authors: | Yoshida, Takayuki Browse this author | | Fukaya, Masahiro Browse this author | | Uchigashima, Motokazu Browse this author | | Miura, Eriko Browse this author | | Kamiya, Haruyuki Browse this author →KAKEN DB | | Kano, Masanobu Browse this author | | Watanabe, Masahiko Browse this author →KAKEN DB |
| Keywords: | Endocannabinoid | | 2-arachidonoyl-glycerol | | 2-AG | | Diacylglycerol lipase | | DAGL | | CB1 | | Immunohistochemistry | | Purkinje cell | | Hippocampal pyramidal cell | | Mouse |
| Issue Date: | 3-May-2006 |
| Publisher: | Society for Neuroscience |
| Journal Title: | The Journal of neuroscience : the official journal of the Society for Neuroscience |
| Volume: | 26 |
| Issue: | 18 |
| Start Page: | 4740 |
| End Page: | 4751 |
| Publisher DOI: | 10.1523/JNEUROSCI.0054-06.2006 |
| PMID: | 16672646 |
| Abstract: | 2-arachidonoyl-glycerol (2-AG) is an endocannabinoid that is released from postsynaptic neurons, acts retrogradely on presynaptic cannabinoid receptor CB1, and induces short- and long-term suppression of transmitter release. To understand the mechanisms of the 2-AG-mediated retrograde modulation, we investigated subcellular localization of a major 2-AG biosynthetic enzyme, diacylglycerol lipase-alpha (DAGLalpha), by using immunofluorescence and immunoelectron microscopy in the mouse brain. In the cerebellum, DAGLalpha was predominantly expressed in Purkinje cells. DAGLalpha was detected on the dendritic surface and occasionally on the somatic surface, with a distal-to-proximal gradient from spiny branchlets toward somata. DAGLalpha was highly concentrated at the base of spine neck and also accumulated with much lower density on somatodendritic membrane around the spine neck. However, DAGLalpha was excluded from the main body of spine neck and head. In hippocampal pyramidal cells, DAGLalpha was also accumulated in spines. In contrast to the distribution in Purkinje cells, DAGLalpha was distributed in the spine head, neck, or both, whereas somatodendritic membrane was labeled very weakly. These results indicate that DAGLalpha is essentially targeted to postsynaptic spines in cerebellar and hippocampal neurons, but its fine distribution within and around spines is differently regulated between the two neurons. The preferential spine targeting should enable efficient 2-AG production on excitatory synaptic activity and its swift retrograde modulation onto nearby presynaptic terminals expressing CB1. Furthermore, different fine localization within and around spines suggests that the distance between postsynaptic 2-AG production site and presynaptic CB1 is differentially controlled depending on neuron types. |
| Relation: | http://www.jneurosci.org/content/26/18/4740.full.pdf+html |
| Type: | article |
| URI: | http://hdl.handle.net/2115/51762 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 渡邉 雅彦
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