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Hokkaido University Collection of Scholarly and Academic Papers >
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Mapping of conserved and species-specific antibody epitopes on the Ebola virus nucleoprotein
| Title: | Mapping of conserved and species-specific antibody epitopes on the Ebola virus nucleoprotein |
| Authors: | Changula, Katendi Browse this author | | Yoshida, Reiko Browse this author | | Noyori, Osamu Browse this author | | Marzi, Andrea Browse this author | | Miyamoto, Hiroko Browse this author | | Ishijima, Mari Browse this author | | Yokoyama, Ayaka Browse this author | | Kajihara, Masahiro Browse this author | | Feldmann, Heinz Browse this author | | Mweene, Aaron S. Browse this author | | Takada, Ayato Browse this author →KAKEN DB |
| Keywords: | Ebola virus | | Nucleoprotein | | Antibody epitope | | Monoclonal antibody | | Synthetic peptide |
| Issue Date: | Sep-2013 |
| Publisher: | Elsevier |
| Journal Title: | Virus Research |
| Volume: | 176 |
| Issue: | 1-2 |
| Start Page: | 83 |
| End Page: | 90 |
| Publisher DOI: | 10.1016/j.virusres.2013.05.004 |
| Abstract: | Filoviruses (viruses in the genus Ebolavirus and Marburgvirus in the family Filoviridae) cause severe haemorrhagic fever in humans and nonhuman primates. Rapid, highly sensitive, and reliable filovirus-specific assays are required for diagnostics and outbreak control. Characterisation of antigenic sites in viral proteins can aid in the development of viral antigen detection assays such immunochromatography-based rapid diagnosis. We generated a panel of mouse monoclonal antibodies (mAbs) to the nucleoprotein (NP) of Ebola virus belonging to the species Zaire ebolavirus. The mAbs were divided into seven groups based on the profiles of their specificity and cross-reactivity to other species in the Ebolavirus genus. Using synthetic peptides corresponding to the Ebola virus NP sequence, the mAb binding sites were mapped to seven antigenic regions in the C-terminal half of the NP, including two highly conserved regions among all five Ebolavirus species currently known. Furthermore, we successfully produced species-specific rabbit antisera to synthetic peptides predicted to represent unique filovirus B-cell epitopes. Our data provide useful information for the development of Ebola virus antigen detection assays. (C) 2013 Elsevier B.V. All rights reserved. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/54786 |
| Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 高田 礼人
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