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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
Polyphosphate accumulation is driven by transcriptome alterations that lead to near-synchronous and near-equivalent uptake of inorganic cations in an arbuscular mycorrhizal fungus
| Title: | Polyphosphate accumulation is driven by transcriptome alterations that lead to near-synchronous and near-equivalent uptake of inorganic cations in an arbuscular mycorrhizal fungus |
| Authors: | Kikuchi, Yusuke Browse this author | | Hijikata, Nowaki Browse this author | | Yokoyama, Kaede Browse this author | | Ohtomo, Ryo Browse this author | | Handa, Yoshihiro Browse this author | | Kawaguchi, Masayoshi Browse this author | | Saito, Katsuharu Browse this author | | Ezawa, Tatsuhiro Browse this author →KAKEN DB |
| Keywords: | arbuscular mycorrhizal (AM) fungi | | inorganic cation | | polyphosphate | | symbiosis | | transcriptome |
| Issue Date: | Nov-2014 |
| Publisher: | Blackwell Publishing |
| Journal Title: | New Phytologist |
| Volume: | 204 |
| Issue: | 3 |
| Start Page: | 638 |
| End Page: | 649 |
| Publisher DOI: | 10.1111/nph.12937 |
| PMID: | 25039900 |
| Abstract: | ・Arbuscular mycorrhizal (AM) fungi accumulate a massive amount of phosphate as polyphosphate to deliver to the host, but the physiological and molecular mechanisms underlying have yet to be elucidated. In the present study, the dynamics of cationic components during polyphosphate accumulation were investigated in conjunction with transcriptome analysis. ・Rhizophagus sp. HR1 was grown with Lotus japonicus under phosphorus-deficient conditions, and extraradical mycelia were harvested after phosphate application at prescribed intervals. Levels of polyphosphate, inorganic cations, and amino acids were measured, and RNA-Seq was performed on the Illumina platform. ・Phosphate application triggered not only polyphosphate accumulation but also near-synchronous and -equivalent uptake of Na+, K+, Ca2+, and Mg2+, whereas no distinct changes in the levels of amino acids were observed. During polyphosphate accumulation, genes responsible for mineral uptake, phosphate and nitrogen metabolisms, and maintenance of cellular homeostasis were up-regulated. ・The results suggest that the inorganic cations play a major role in neutralizing negative charge of polyphosphate, and these processes are achieved by the orchestrated regulation of gene expression. Our findings for the first time provide a global picture of the cellular responses to increased phosphate availability, which is the initial process of nutrient delivery in the associations. |
| Rights: | The definitive version is available at www.blackwellsynergy.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/58046 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 江澤 辰広
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