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Hokkaido University Collection of Scholarly and Academic Papers >
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LIM-homeodomain transcription factor Awh is a key component activating all three fibroin genes, fibH, fibL and fhx, in the silk gland of the silkworm, Bombyx mori
| Title: | LIM-homeodomain transcription factor Awh is a key component activating all three fibroin genes, fibH, fibL and fhx, in the silk gland of the silkworm, Bombyx mori |
| Authors: | Kimoto, Mai Browse this author | | Tsubota, Takuya Browse this author | | Uchino, Keiro Browse this author | | Sezutsu, Hideki Browse this author →KAKEN DB | | Takiya, Shigeharu Browse this author →KAKEN DB |
| Keywords: | Bombyx mori | | Silk gland | | Fibroin | | Awh | | Transgenic |
| Issue Date: | Jan-2015 |
| Publisher: | Elsevier |
| Journal Title: | Insect biochemistry and molecular biology |
| Volume: | 56 |
| Start Page: | 29 |
| End Page: | 35 |
| Publisher DOI: | 10.1016/j.ibmb.2014.11.003 |
| PMID: | 25449130 |
| Abstract: | In the silkworm Bombyx mori, three fibroin genes, fibroin-heavy-chain (fibH), fibroin-light-chain (fibL) and fibrohexamerin (fhx), are coexpressed only in the posterior silk gland (PSG) cells, while the sericin genes encoding silk glue proteins are expressed in the middle silk gland (MSG) cells. Silk gland factor-2 (SGF-2) is a PSG-specific activator complex of fibH, composed of a LIM-homeodomain protein, Awh, and its cofactors, Ldb and Lcaf. We investigated whether SGF-2 can activate other fibroin genes using transgenic silkworms. The genes for Ldb and Lcaf were expressed ubiquitously in various tissues, while the gene for Awh was expressed strictly specific in PSG of the wild type silkworms. Misexpression of Awh in transgenic silkworms induced ectopic expression of fibL and fhx as well as fibH in MSG. Coincidently with the induction of fibL and fhx by Awh, binding of SGF-2 to the promoter of fibL and fhx was detected in vitro, and SGF-2 binds directly to the fhx core promoter. Ectopic expression of the fibroin genes was observed at high levels in the middle part of MSG. Moreover, fibL and fhx were induced in the anterior silk gland (ASG) of the transgenic silkworms, but fibH was not. These results indicate that Awh is a key activator of all three fibroin genes, and the activity is probably regulated in conjunction with additional factors. |
| Rights: | (C) 2014 Elsevier Ltd. All rights reserved. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/58056 |
| Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 瀧谷 重治
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