| Title: | Bone augmentation using a highly porous PLGA/β-TCP scaffold containing fibroblast growth factor-2 |
| Authors: | Yoshida, Takashi Browse this author |
| Miyaji, Hirofumi Browse this author →KAKEN DB |
| Otani, Kaori Browse this author |
| Inoue, Kana Browse this author |
| Nakane, Kazuyasu Browse this author |
| Nishimura, Hiroyuki Browse this author |
| Ibara, Asako Browse this author |
| Shimada, Ayumu Browse this author |
| Ogawa, Kosuke Browse this author |
| Nishida, Erika Browse this author |
| Sugaya, Tsutomu Browse this author →KAKEN DB |
| Sun, Ling Browse this author |
| Fugetsu, Bunshi Browse this author →KAKEN DB |
| Kawanami, Masamitsu Browse this author →KAKEN DB |
| Keywords: | β-tricalcium phosphate (β-TCP) |
| co-poly lactic acid/glycolic acid (PLGA) |
| fibroblast growth factor-2 (FGF2) |
| bone tissue engineering |
| Issue Date: | May-2015 |
| Publisher: | Wiley-Blackwell |
| Journal Title: | Journal of Periodontal Research |
| Volume: | 50 |
| Issue: | 2 |
| Start Page: | 265 |
| End Page: | 273 |
| Publisher DOI: | 10.1111/jre.1 2206 |
| PMID: | 24966062 |
| Abstract: | Background and objective: β-tricalcium phosphate (β-TCP), a bio-absorbable ceramic, facilitates bone conductivity. We constructed a highly porous three dimensional scaffold using β-TCP for bone tissue engineering and coated it with co-poly lactic acid/glycolic acid (PLGA) to improve the mechanical strength and biological performance. The aim of this study was to examine the effect of the implantation of the PLGA/β-TCP scaffold loaded with fibroblast growth factor-2 (FGF2) on bone augmentation.
Material and methods: The β-TCP scaffold was fabricated by the replica method using polyurethane foam, then coated with PLGA. The PLGA/β-TCP scaffold was characterized by SEM, TEM, XRD, compressive testing, cell culture, and a subcutaneous implant test. Subsequently, a bone forming test was performed using fifty two rats. The β-TCP scaffold, PLGA-coated scaffold, and β-TCP scaffold and PLGA-coated scaffolds loaded with FGF2, were implanted into rat cranial bone. Histological observations were made at 10 and 35 days post-surgery.
Results: SEM and TEM observations showed a thin PLGA layer on the β-TCP particles after coating. High porosity of the scaffold was exhibited after PLGA coating (> 90%), and the compressive strength of the PLGA/β-TCP scaffold was 6-fold greater than the non-coated scaffold. Good biocompatibility of the PLGA/β-TCP scaffold was found in the culture and implant tests. Histological samples obtained following implantation of PLGA/β-TCP scaffold loaded with FGF2 showed significant bone augmentation.
Conclusion: The PLGA coating improved the mechanical strength of β-TCP scaffolds while maintaining high porosity and tissue compatibility. PLGA/β-TCP scaffolds in combination with FGF2 are bioeffective for bone augmentation. |
| Rights: | This is the peer reviewed version of the following article: [Bone augmentation using a highly porous PLGA/β-TCP scaffold containing fibroblast growth factor-2], which has been published in final form at [http://dx.doi.org/10.1111/jre.1 2206]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/61984 |
| Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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