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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
Up-regulation of genes involved in N-acetylglucosamine uptake and metabolismsuggests a recycling mode of chitin in intraradical mycelium of arbuscular mycorrhizal fungi
| Title: | Up-regulation of genes involved in N-acetylglucosamine uptake and metabolismsuggests a recycling mode of chitin in intraradical mycelium of arbuscular mycorrhizal fungi |
| Authors: | Kobae, Yoshihiro Browse this author | | Kawachi, Miki Browse this author | | Saito, Katsuharu Browse this author | | Kikuchi, Yusuke Browse this author | | Ezawa, Tatsuhiro Browse this author →KAKEN DB | | Maeshima, Masayoshi Browse this author | | Hata, Shingo Browse this author | | Fujiwara, Toru Browse this author |
| Keywords: | Arbuscular mycorrhizal fungi | | Chitin recycling | | N-acetylglucosamine (GlcNAc) transporter | | Rhizophagus irregularis |
| Issue Date: | Jul-2015 |
| Publisher: | springer |
| Journal Title: | Mycorrhiza |
| Volume: | 25 |
| Issue: | 5 |
| Start Page: | 411 |
| End Page: | 417 |
| Publisher DOI: | 10.1007/s00572-014-0623-2 |
| Abstract: | Arbuscular mycorrhizal (AM) fungi colonize roots and form two kinds of mycelium, intraradical mycelium (IRM) and extraradical mycelium (ERM). Arbuscules are characteristic IRM structures that highly branch within host cells in order to mediate resource exchange between the symbionts. They are ephemeral structures and at the end of their life span, arbuscular branches collapse from the tip, fungal cytoplasm withdraws, and the whole arbuscule shrinks into fungal clumps. The exoskeleton of an arbuscule contains structured chitin, which is a polymer of N-acetylglucosamine (GlcNAc), whereas a collapsed arbuscule does not. The molecular mechanisms underlying the turnover of chitin in AM fungi remain unknown. Here, a GlcNAc transporter, RiNGT, was identified from the AM fungus Rhizophagus irregularis. Yeast mutants defective in endogenous GlcNAc uptake and expressing RiNGT took up 14C-GlcNAc, and the optimum uptake was at acidic pH values (pH 4.0–4.5). The transcript levels of RiNGT in IRM in mycorrhizal Lotus japonicus roots were over 1000 times higher than those in ERM. GlcNAc-6-phosphate deacetylase (DAC1) and glucosamine-6-phosphate isomerase (NAG1) genes, which are related to the GlcNAc catabolism pathway, were also induced in IRM. Altogether, data suggest the existence of an enhanced recycling mode of GlcNAc in IRM of AM fungi. |
| Rights: | The original publication is available at www.springerlink.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/62598 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 江澤 辰広
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