| Title: | Two male sterility-inducing cytoplasms of beet (Beta vulgaris) are genetically distinct but have closely related mitochondrial genomes: implication of a substoichiometric mitochondrial DNA molecule in their evolution |
| Authors: | Onodera, Yasuyuki Browse this author |
| Arakawa, Takumi Browse this author |
| Yui-Kurino, Rika Browse this author |
| Yamamoto, Masayuki P Browse this author |
| Kitazaki, Kazuyoshi Browse this author |
| Ebe, Shigehiko Browse this author |
| Matsunaga, Muneyuki Browse this author |
| Taguchi, Kazunori Browse this author |
| Kuroda, Yosuke Browse this author |
| Yamashita, Shiko Browse this author |
| Sakai, Tomoyuki Browse this author |
| Kinoshita, Toshiro Browse this author →KAKEN DB |
| Mikami, Tetsuo Browse this author →KAKEN DB |
| Kubo, Tomohiko Browse this author →KAKEN DB |
| Keywords: | F1 hybrid |
| mitochondrial evolution |
| mitochondrial gene |
| nuclear-cytoplasmic interaction |
| plant mitochondria |
| substoichiometric DNA |
| Issue Date: | Nov-2015 |
| Publisher: | Springer |
| Journal Title: | Euphytica |
| Volume: | 206 |
| Issue: | 2 |
| Start Page: | 365 |
| End Page: | 379 |
| Publisher DOI: | 10.1007/s10681-015-1484-2 |
| Abstract: | I-12CMS(2) and I-12CMS(3) are sugar beet lines with different sources of cytoplasmic male sterility (CMS) derived from wild beets in Turkey and Pakistan, respectively. We established that I-12CMS(2) has a genetically distinct cytoplasm, but its mitochondrial genome is very similar to I-12CMS(3). Male fertility was assessed in F1 hybrids produced with a common pollen parent. Fertility in the F1's carrying the I-12CMS(3) cytoplasm exceeded that of the F1's with the I-12CMS(2) cytoplasm. Organization of the I-12CMS(2) and I-12CMS(3) mitochondrial genomes were compared based on their physical maps. Mitochondrial genomes of the two strains were largely collinear, except for a large deletion in the noncoding region of I-12CMS(2). Because a mitochondrial orf129 in the I-12CMS(3) cytoplasm is associated with a male sterility phenotype and preservation of orf129 was evident in I-12CMS(2), I-12CMS(2) orf129 was investigated in detail. I-12CMS(2) plants contained three to five times more ORF129 protein than did I-12CMS(3) plants. A single nucleotide substitution, present in the putative promoter region of orf129, appeared to be responsible for the differential accumulation of orf129 transcript. A long N-terminal extension of atp6 is a common feature of some beet CMSs and is found in I-12CMS(2), but the amino acid sequence is unique. I-12CMS(3) mitochondria, but not I-12CMS(2) mitochondria, were found to be heteroplasmic. This heteroplasmy is characterized by a substoichiometric DNA molecule(s) that has at least two I-12CMS(2)-type mitochondrial loci, suggesting the possibility that the I-12CMS(2) mitochondrial genome might have evolved from such a substoichiometric DNA molecule in I-12CMS(3) mitochondria. |
| Rights: | The final publication is available at link.springer.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/63730 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
