| Title: | Serotyping dengue virus with isothermal amplification and a portable sequencer |
| Authors: | Yamagishi, Junya Browse this author →KAKEN DB |
| Runtuwene, Lucky R. Browse this author |
| Hayashida, Kyoko Browse this author |
| Mongan, Arthur E. Browse this author |
| Lan Anh Nguyen Thi Browse this author |
| Linh Nguyen Thuy Browse this author |
| Cam Nguyen Nhat Browse this author |
| Limkittikul, Kriengsak Browse this author |
| Sirivichayakul, Chukiat Browse this author |
| Sathirapongsasuti, Nuankanya Browse this author |
| Frith, Martin Browse this author |
| Makalowski, Wojciech Browse this author |
| Eshita, Yuki Browse this author |
| Sugano, Sumio Browse this author |
| Suzuki, Yutaka Browse this author |
| Issue Date: | 14-Jun-2017 |
| Publisher: | Nature Publishing Group |
| Journal Title: | Scientific reports |
| Volume: | 7 |
| Start Page: | 3510 |
| Publisher DOI: | 10.1038/s41598-017-03734-5 |
| Abstract: | The recent development of a nanopore-type portable DNA sequencer has changed the way we think about DNA sequencing. We can perform sequencing directly in the field, where we collect the samples. Here, we report the development of a novel method to detect and genotype tropical disease pathogens, using dengue fever as a model. By combining the sequencer with isothermal amplification that only requires a water bath, we were able to amplify and sequence target viral genomes with ease. Starting from a serum sample, the entire procedure could be finished in a single day. The analysis of blood samples collected from 141 Indonesian patients demonstrated that this method enables the clinical identification and serotyping of the dengue virus with high sensitivity and specificity. The overall successful detection rate was 79%, and a total of 58 SNVs were detected. Similar analyses were conducted on 80 Vietnamese and 12 Thai samples with similar performance. Based on the obtained sequence information, we demonstrated that this approach is able to produce indispensable information for etiologically analyzing annual or regional diversifications of the pathogens. |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/67101 |
| Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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