|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Engineering / Faculty of Engineering >
Peer-reviewed Journal Articles, etc >
Bacterial histo-blood group antigens contributing to genotype-dependent removal of human noroviruses with a microfiltration membrane
This item is licensed under:Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
| Title: | Bacterial histo-blood group antigens contributing to genotype-dependent removal of human noroviruses with a microfiltration membrane |
| Authors: | Amarasiri, Mohan Browse this author | | Hashiba, Satoshi Browse this author | | Miura, Takayuki Browse this author | | Nakagomi, Toyoko Browse this author →KAKEN DB | | Nakagomi, Osamu Browse this author →KAKEN DB | | Ishii, Satoshi Browse this author | | Okabe, Satoshi Browse this author →KAKEN DB | | Sano, Daisuke Browse this author →KAKEN DB |
| Keywords: | Extracellular polymeric substance | | Histo-blood group antigen | | Human norovirus | | Lipopolysaccharide | | Microfiltration | | Membrane bioreactor |
| Issue Date: | 15-May-2016 |
| Publisher: | Elsevier |
| Journal Title: | Water research |
| Volume: | 95 |
| Start Page: | 383 |
| End Page: | 391 |
| Publisher DOI: | 10.1016/j.watres.2016.04.018 |
| PMID: | 27095709 |
| Abstract: | We demonstrated the genotype-dependent removal of human norovirus particles with a microfiltration (MF) membrane in the presence of bacteria bearing histo-blood group antigens (HBGAs). Three genotypes (GII.3, GII.4, and GII.6) of norovirus-like particles (NoVLPs) were mixed with three bacterial strains (Enterobacter sp. SENG-6, Escherichia coli 086:K61:B7, and Staphylococcus epidermidis), respectively, and the mixture was filtered with an MF membrane having a nominal pore size of 0.45 mu m. All NoVLP genotypes were rejected by the MF membrane in the presence of Enterobacter sp. SENG-6, which excreted HBGAs as extracellular polymeric substances (EPS). This MF membrane removal of NoVLPs was not significant when EPS was removed from cells of Enterobacter sp. SENG-6. GII.6 NoVLP was not rejected with the MF membrane in the presence of E. coli 086:K61:B7, but the removal of EPS of E. coli 086:K61:B7 increased the removal efficiency due to the interaction of NoVLPs with the exposed B-antigen in lipopolysaccharide (LPS) of E. coli 086:K61:B7. No MF membrane removal of all three genotypes was observed when S. epidermidis, an HBGA-negative strain, was mixed with NoVLPs. These results demonstrate that the location of HBGAs on bacterial cells is an important factor in determining the genotype-dependent removal efficiency of norovirus particles with the MF membrane. The presence of HBGAs in mixed liquor suspended solids from a membrane bioreactor (MBR) pilot plant was confirmed by immune-transmission electron microscopy, which implies that bacterial HBGAs can contribute to the genotype-dependent removal of human noroviruses with MBR using MF membrane. |
| Rights: | © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/70214 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 佐野 大輔
|
