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Hokkaido University Collection of Scholarly and Academic Papers >
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Infection of Epstein–Barr Virus in Type III Latency Modulates Biogenesis of Exosomes and the Expression Profile of Exosomal miRNAs in the Burkitt Lymphoma Mutu Cell Lines
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| Title: | Infection of Epstein–Barr Virus in Type III Latency Modulates Biogenesis of Exosomes and the Expression Profile of Exosomal miRNAs in the Burkitt Lymphoma Mutu Cell Lines |
| Authors: | Nanbo, Asuka Browse this author →KAKEN DB | | Katano, Harutaka Browse this author | | Kataoka, Michiyo Browse this author | | Hoshina, Shiho Browse this author | | Sekizuka, Tsuyoshi Browse this author | | Kuroda, Makoto Browse this author | | Ohba, Yusuke Browse this author →KAKEN DB |
| Keywords: | Epstein–Barr virus | | Burkitt’s lymphoma | | exosomes | | miRNA | | next-generation sequencing |
| Issue Date: | Jul-2018 |
| Publisher: | MDPI |
| Journal Title: | Cancers |
| Volume: | 10 |
| Issue: | 7 |
| Start Page: | 237 |
| Publisher DOI: | 10.3390/cancers10070237 |
| Abstract: | Infection of Epstein-Barr virus (EBV), a ubiquitous human gamma herpesvirus, is associated with various malignancies in B lymphocytes and epithelial cells. EBV encodes 49 microRNAs in two separated regions, termed the BART and BHRF1 loci. Although accumulating evidence demonstrates that EBV infection regulates the profile of microRNAs in the cells, little is known about the microRNAs in exosomes released from infected cells. Here, we characterized the expression profile of intracellular and exosomal microRNAs in EBV-negative, and two related EBV-infected Burkitt lymphoma cell lines having type I and type III latency by next-generation sequencing. We found that the biogenesis of exosomes is upregulated in type III latently infected cells compared with EBV-negative and type I latently infected cells. We also observed that viral and several specific host microRNAs were predominantly incorporated in the exosomes released from the cells in type III latency. We confirmed that multiple viral microRNAs were transferred to the epithelial cells cocultured with EBV-infected B cells. Our findings indicate that EBV infection, in particular in type III latency, modulates the biogenesis of exosomes and the profile of exosomal microRNAs, potentially contributing to phenotypic changes in cells receiving these exosomes. |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/71077 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 南保 明日香
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