Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Engineering / Faculty of Engineering >
Peer-reviewed Journal Articles, etc >
An Electrochemical Sensor Based on Structure Switching of Dithiol-modified Aptamer for Simple Detection of Ochratoxin A
Title: | An Electrochemical Sensor Based on Structure Switching of Dithiol-modified Aptamer for Simple Detection of Ochratoxin A |
Authors: | Mazaafrianto, Donny Nugraha Browse this author | Ishida, Akihiko Browse this author →KAKEN DB | Maeki, Masatoshi Browse this author | Tani, Hirofumi Browse this author →KAKEN DB | Tokeshi, Manabu Browse this author →KAKEN DB |
Keywords: | Dithiol | microfabricated device | electrochemical detection | aptamer | methylene blue | ochratoxin A | signal-on |
Issue Date: | 10-Nov-2019 |
Publisher: | Japan Society for Analytical Chemistry |
Journal Title: | Analytical sciences |
Volume: | 35 |
Issue: | 11 |
Start Page: | 1221 |
End Page: | 1226 |
Publisher DOI: | 10.2116/analsci.19P240 |
Abstract: | In this study, we developed an electrochemical sensor for ochratoxin A (OTA) by using an aptamer having a dithiol-based anchor, which exhibited higher stability on a gold electrode than a monothiol-based aptamer because of its two anchors. The sensor was also based on a signal-on scheme that produces a signal current resulting from structure-switching of the aptamer upon interaction with OTA. For simple fabrication of this sensor, the non-covalent interaction of methylene blue with the aptamer was also employed as an electrochemical indicator. In this study, the performance of the sensor, including the dissociation constant of the aptamer-OTA complex, was characterized. The proposed sensor exhibited high reproducibility and enough sensitivity to detect the minimum amount of OTA required for the analysis of real food samples with a limit of detection of 113 pM. |
Type: | article |
URI: | http://hdl.handle.net/2115/76500 |
Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
|