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Enzymatic production of xylooligosaccharides from red alga dulse (Palmaria sp.) wasted in Japan
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| Title: | Enzymatic production of xylooligosaccharides from red alga dulse (Palmaria sp.) wasted in Japan |
| Authors: | Yamamoto, Yohei Browse this author | | Kishimura, Hideki Browse this author →KAKEN DB | | Kinoshita, Yasunori Browse this author | | Saburi, Wataru Browse this author →KAKEN DB | | Kumagai, Yuya Browse this author →KAKEN DB | | Yasui, Hajime Browse this author →KAKEN DB | | Ojima, Takao Browse this author →KAKEN DB |
| Keywords: | Xylooligosaccharides | | Red alga | | Palmaria sp. | | Xylan | | Hemicellulase |
| Issue Date: | Jul-2019 |
| Publisher: | Elsevier |
| Journal Title: | Process biochemistry |
| Volume: | 82 |
| Start Page: | 117 |
| End Page: | 122 |
| Publisher DOI: | 10.1016/j.procbio.2019.03.030 |
| Abstract: | Red alga dulse (Palmaria sp.) has xylan with a small amount of cellulose and less lignin, hence these characteristics may provide some advantages in the enzymatic production of xylooligosaccharides (XOS). To evaluate dulse xylan as XOS source, we prepared xylan rich fraction (DXRF) and attempted XOS production using commercial enzymes. 13.8% of xylan rich fraction (DXRF: containing 52.2% of xylan) was obtained from dried dulse powder. We then evaluated the hydrolysis products of two commercial enzymes, revealing that hydrolysate of Hemicellulase amano 90 contained less xylose. The effective XOS production by the enzyme was evaluated in terms of enzyme dose, DXRF concentration and reaction period, showing that 66.6% of XOS was obtained from DXRF with the hydrolysis rate of 82% under the following condition: 54 U Hemicellulase amano 90, 10 mg/ml DXRF, pH 4.5, 50 degrees C for 24 h. These results indicated that red algae xylan is suitable for XOS source. |
| Rights: | © 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/ | | https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/78807 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 岸村 栄毅
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