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A Novel Metal Adsorbent Composed of a Hexa-histidine Tag and a Carbohydrate-binding Module on Cellulose
| Title: | A Novel Metal Adsorbent Composed of a Hexa-histidine Tag and a Carbohydrate-binding Module on Cellulose |
| Authors: | Togo, Yuki Browse this author | | Nakashima, Kazunori Browse this author | | Mwandira, Wilson Browse this author | | Kawasaki, Satoru Browse this author →KAKEN DB |
| Keywords: | Carbohydrate-binding module | | immobilization | | metal affinity tag | | transition metals |
| Issue Date: | Apr-2020 |
| Publisher: | 日本分析化学会（Japan Society for Analytical Chemistry） |
| Journal Title: | Analytical sciences |
| Volume: | 36 |
| Issue: | 4 |
| Start Page: | 459 |
| End Page: | 464 |
| Publisher DOI: | 10.2116/analsci.19P356 |
| Abstract: | We developed a novel metal adsorbent composed of bio-based materials, cellulose and a protein. The approach involved the immobilization of a hexa-histidine tag (His(6)), which shows an affinity for an intermediate acid (metal ion) in Hard and Soft Acids and Bases (HSAB) theory, on cellulose by fusing with a carbohydrate-binding module (CBM). The results show that CBM-His(6)-bound cellulose has adsorption selectivity reflecting the original properties of His(6). Additionally, we prepared three configurations of CBM-His(6) proteins, which were subsequently immobilized on filter paper for Ni2+ ion adsorption. Of these configurations, we found that the protein containing two His(6) tags at each terminus (N- and C-) of CBM exhibited the highest metal adsorption ability. Furthermore, XPS analysis confirmed the binding of Ni2+ ions on the cellulose. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/78992 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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