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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Environmental Science / Faculty of Environmental Earth Science >
Peer-reviewed Journal Articles, etc >
Highly sensitive detection of clenbuterol in urine sample by using surface plasmon resonance immunosensor
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| Title: | Highly sensitive detection of clenbuterol in urine sample by using surface plasmon resonance immunosensor |
| Authors: | Kabiraz, Dulal C. Browse this author | | Morita, Kinichi Browse this author | | Sakamoto, Kazuhira Browse this author | | Takahashi, Masashi Browse this author →KAKEN DB | | Kawaguchi, Toshikazu Browse this author →KAKEN DB |
| Keywords: | Clenbuterol | | SPR immunosensing | | Indirect competitive inhibition | | Inhibitor determination | | Urine |
| Issue Date: | 15-Aug-2018 |
| Publisher: | Elsevier |
| Journal Title: | Talanta |
| Volume: | 186 |
| Start Page: | 521 |
| End Page: | 526 |
| Publisher DOI: | 10.1016/j.talanta.2018.04.011 |
| Abstract: | This study proposed the filtration method for removal of inhibitors from real urine samples for immunoassay without centrifuge. Although the inhibitors could not be removed by the physical filtration, the carboxyl group terminated silica effectively removed the inhibitors. In a low pH, antibody formed aggregation due to the protonation. We propose to adjust pH of the sample solution by adding a phosphate buffer solution (pH 7.5). As a result of pretreatment, the SPR immunosensing achieved the SPR signal of 45 mdeg and a low limit of detection with 100 ppq (100 fg mL(-1)). |
| Rights: | © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/79063 |
| Appears in Collections: | 国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 川口 俊一
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