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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Dental Medicine / Faculty of Dental Medicine >
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Histochemical examination on principal collagen fibers in periodontal ligaments of ascorbic acid-deficient ODS-od/od rats
| Title: | Histochemical examination on principal collagen fibers in periodontal ligaments of ascorbic acid-deficient ODS-od/od rats |
| Authors: | Hasegawa, Tomoka Browse this author →KAKEN DB | | Miyamoto-Takasaki, Yukina Browse this author | | Abe, Miki Browse this author | | Qiu, Zixuan Browse this author | | Yamamoto, Tomomaya Browse this author | | Yimin Browse this author | | Yoshida, Taiji Browse this author | | Yoshino, Hirona Browse this author | | Hongo, Hiromi Browse this author →KAKEN DB | | Yokoyama, Ayako Browse this author | | Sasaki, Muneteru Browse this author | | Kuroshima, Shinichiro Browse this author | | Hara, Kuniko Browse this author | | Kobayashi, Masatoshi Browse this author | | Akiyama, Yasuhiro Browse this author | | Maeda, Takeyasu Browse this author | | Luiz de Freitas, Paulo Henrique Browse this author | | Li, Minqi Browse this author | | Amizuka, Norio Browse this author →KAKEN DB |
| Keywords: | periodontal ligament | | ascorbic acid | | collagen fibril | | reticular fibril | | histochemistry |
| Issue Date: | 9-Oct-2019 |
| Publisher: | Oxford University Press |
| Journal Title: | Microscopy |
| Volume: | 68 |
| Issue: | 5 |
| Start Page: | 349 |
| End Page: | 358 |
| Publisher DOI: | 10.1093/jmicro/dfz021 |
| Abstract: | In this study, we aimed to clarify the role of ascorbic acid in collagen synthesis in periodontal ligaments using osteogenic disorder Shionogi (ODS)/ShiJcl-od/od rats lacking L-gulonolactone oxidase. These rats cannot synthesize ascorbic acid in vivo. Eight-week-old ODS/ShiJcl-od/od male rats were administered ascorbic acid solution at a concentration of 200 mg/dL (control group, n = 6) or ascorbic acid solution at concentration of 0.3 mg/dL (insufficient group, n = 12). Six rats of the insufficient group were then given with ascorbic acid solution at concentration of 200 mg/dL for additional 3 weeks (rescued group, n = 6), and then, their mandibles were histochemically examined. Consequently, the insufficient group specimens were seen to possess fewer collagen fibers, and silver impregnation revealed numerous fine, reticular fiber-like fibrils branching off from collagen in the periodontal ligaments. In control group, faint immunoreactivities for matrix metalloproteinase (MMP)2 and cathepsin H were seen in the periphery of blood vessels and throughout the ligament, respectively. In contrast, in the insufficient group, intense MMP2-immunoreactivity was observed to be associated with collagen fibrils in the periodontal ligaments, and cathepsin H-immunopositivity was seen in ligamentous cells. The rescued group showed abundant collagen fibers filling the periodontal ligament space. Under transmission electron microscopy, ligamentous fibroblasts incorporated collagen fibrils into tubular endosomes/lysosomes while simultaneously synthesizing collagen fibril bundles. Thus, ascorbic acid insufficiency affected the immunolocalization of cathepsin H and MMP2; however, ligamentous fibroblasts appear to possess the potential to synthesize collagen fibers when supplied with ascorbic acid. |
| Rights: | This is a pre-copyedited, author-produced version of an article accepted for publication in [Microscopy] following peer review. The version of record [Hasegawa, Tomoka; Miyamoto-Takasaki, Yukina; Abe, Miki; Qiu, Zixuan; Yamamoto, Tomomaya; Yimin; Yoshida, Taiji; Yoshino, Hirona; Hongo, Hiromi; Yokoyama, Ayako; Sasaki, Muneteru; Kuroshima, Shinichiro; Hara, Kuniko; Kobayashi, Masatoshi; Akiyama, Yasuhiro; Maeda, Takeyasu; Luiz de Freitas, Paulo Henrique; Li, Minqi; Amizuka, Norio; Histochemical examination on principal collagen fibers in periodontal ligaments of ascorbic acid-deficient ODS-od/od rats. Microscopy, Volume 68, Issue 5, 9 October 2019, Pages 349–358] is available online at: [https://doi.org/10.1093/jmicro/dfz021]. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/79521 |
| Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 長谷川 智香
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