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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Dental Medicine / Faculty of Dental Medicine >
Peer-reviewed Journal Articles, etc >
Antibacterial coating of tooth surface with ion-releasing pre-reacted glass-ionomer (S-PRG) nanofillers
This item is licensed under:Creative Commons Attribution 4.0 International
| Title: | Antibacterial coating of tooth surface with ion-releasing pre-reacted glass-ionomer (S-PRG) nanofillers |
| Authors: | Mayumi, Kayoko Browse this author | | Miyaji, Hirofumi Browse this author →KAKEN DB | | Miyata, Saori Browse this author | | Nishida, Erika Browse this author →KAKEN DB | | Furihata, Tomokazu Browse this author | | Kanemoto, Yukimi Browse this author | | Sugaya, Tsutomu Browse this author →KAKEN DB | | Shitomi, Kanako Browse this author →KAKEN DB | | Akasaka, Tsukasa Browse this author →KAKEN DB |
| Keywords: | Actinomyces naeslundii | | Animal model | | Antibacterial activity | | Anti-inflammatory | | Class II furcation defect | | Ion-releasing | | Periodontitis | | Root dentin | | Streptococcus mutans | | Surface pre-reacted glass-ionomer (S-PRG) filler |
| Issue Date: | Feb-2021 |
| Publisher: | Elsevier |
| Journal Title: | Heliyon |
| Volume: | 7 |
| Issue: | 2 |
| Start Page: | e06147 |
| Publisher DOI: | 10.1016/j.heliyon.2021.e06147 |
| Abstract: | Objectives Surface pre-reacted glass-ionomer (S-PRG) fillers release antibacterial borate and fluoride ions. We fabricated nanoscale S-PRG fillers (S-PRG nanofillers) for antibacterial coating of tooth surfaces and assessed the antibacterial effects of this coating in vitro. In addition, we creating a canine model of periodontitis to evaluate the effectiveness of S-PRG nanofiller application on tooth roots and improvement of periodontal parameters. Methods Human dentin blocks were coated with S-PRG nanofiller (average particle size: 0.48 μm) and then characterized by scanning electron microscopy (SEM), energy dispersive X-ray spectrometer (EDX), and ion-releasing test. Antibacterial effects of dentin blocks coated with S-PRG nanofiller were examined using bacterial strains, Streptococcus mutans and Actinomyces naeslundii. Next, we created an experimental model of periodontitis in furcation of premolars of beagle dogs. Then, S-PRG nanofiller coating was applied onto exposed tooth root surfaces. Periodontal parameters, gingival index (GI), bleeding on probing (BOP), probing pocket depth (PPD), and clinical attachment level (CAL), were measured from baseline until 4 weeks. In addition, bone healing was radiographically and histologically examined. Results SEM and EDX revealed that S-PRG nanofillers uniformly covered the dentin surface after coating. Dentin blocks coated with S-PRG nanofiller showed ion-releasing property, bacterial growth inhibition, and sterilization effects. In the experimental periodontitis model, S-PRG nanofiller coating significantly reduced clinical inflammatory parameters, such as GI (P < 0.01) and BOP (P < 0.05), compared to uncoated samples. In addition, PPD and CAL significantly decreased by S-PRG nanofiller coating (2 weeks: P < 0.05; 3 and 4 weeks: P < 0.01), suggesting the improvement of periodontitis. Micro-CT and histology revealed that bone healing of furcation defects was enhanced by S-PRG nanofiller coating. Conclusion S-PRG nanofiller coating provides antibacterial effects to tooth surfaces and improves clinical parameters of periodontitis. |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/80382 |
| Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 宮治 裕史
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