|
|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
BXSB/MpJ-Yaa mouse model of systemic autoimmune disease shows increased apoptotic germ cells in stage XII of the seminiferous epithelial cycle
| Title: | BXSB/MpJ-Yaa mouse model of systemic autoimmune disease shows increased apoptotic germ cells in stage XII of the seminiferous epithelial cycle |
| Authors: | Otani, Yuki Browse this author | | Ichii, Osamu Browse this author →KAKEN DB | | Masum, Abdul Browse this author | | Kimura, Junpei Browse this author | | Nakamura, Teppei Browse this author | | Elewa, Yaser Hosny Ali Browse this author →ORCID | | Kon, Yasuhiro Browse this author →KAKEN DB |
| Keywords: | Systemic autoimmune disease | | Male infertility | | Spermatogenesis | | Apoptosis | | Meiosis |
| Issue Date: | 4-Apr-2020 |
| Publisher: | Springer |
| Journal Title: | Cell and tissue research |
| Volume: | 381 |
| Start Page: | 203 |
| End Page: | 216 |
| Publisher DOI: | 10.1007/s00441-020-03190-0 |
| Abstract: | In mammals, the reproductive system and autoimmunity regulate mutual functions. Importantly, systemic autoimmune diseases are thought to cause male infertility but the underlying pathological mechanism remains unclear. In this study, the morpho-function of the testes in BXSB/MpJ-Yaa mice was analyzed as a representative mouse model for systemic autoimmune diseases to investigate the effect of excessive autoimmunity on spermatogenesis. At 12 and 24 weeks of age, BXSB/MpJ-Yaa mice showed splenomegaly and increased levels of serum autoantibodies, whereas no controls showed a similar autoimmune condition. In histological analysis, the enlarged lumen of the seminiferous tubules accompanied with scarce spermatozoa in the epididymal ducts were observed in some of the BXSB/MpJ-Yaa and BXSB/MpJ mice but not in C57BL/6N mice. Histoplanimetrical analysis revealed significantly increased residual bodies and apoptotic germ cells in the seminiferous tubules in BXSB/MpJ-Yaa testes without apparent inflammation. Notably, in stage XII of the seminiferous epithelial cycles, the apoptotic germ cell number was remarkably increased, showing a significant correlation with the indices of systemic autoimmune disease in BXSB/MpJ-Yaa mice. Furthermore, the Sertoli cell number was reduced at the early disease stage, which likely caused subsequent morphological changes in BXSB/MpJ-Yaa testes. Thus, our histological study revealed the altered morphologies of BXSB/MpJ-Yaa testes, which were not observed in controls and statistical analysis suggested the effects of an autoimmune condition on this phenotype, particularly the apoptosis of meiotic germ cells. BXSB/MpJ-Yaa mice were shown to be an efficient model to study the relationship between systemic autoimmune disease and the local reproductive system. |
| Rights: | This is a post-peer-review, pre-copyedit version of an article published in Cell and tissue research. The final authenticated version is available online at: http://doi.org/10.1007/s00441-020-03190-0 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/80847 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 昆 泰寛
|
