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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
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Urinary free metanephrines measurement in dogs with adrenal gland diseases using a new simple liquid chromatography tandem mass spectrometry method
| Title: | Urinary free metanephrines measurement in dogs with adrenal gland diseases using a new simple liquid chromatography tandem mass spectrometry method |
| Authors: | Sasaki, Noboru Browse this author →KAKEN DB | | Ikenaka, Yoshinori Browse this author →KAKEN DB | | Inoue, Yumiko Browse this author | | Ichise, Takahiro Browse this author | | Nagata, Noriyuki Browse this author | | Ishizuka, Mayumi Browse this author →KAKEN DB | | Nakayama, Syouta Mm Browse this author | | Nakamura, Kensuke Browse this author →KAKEN DB | | Takiguchi, Mitsuyoshi Browse this author →KAKEN DB |
| Keywords: | canine | | liquid chromatography tandem mass spectrometry | | normetanephrine | | pheochromocytoma | | urinary metanephrines |
| Issue Date: | 5-May-2021 |
| Publisher: | 公益社団法人 日本獣医学会 (The Japanese Society of Veterinary Science) |
| Journal Title: | Journal of veterinary medical science |
| Volume: | 83 |
| Issue: | 4 |
| Start Page: | 648 |
| End Page: | 655 |
| Publisher DOI: | 10.1292/jvms.20-0508 |
| Abstract: | Measurement of urinary metanephrines in spot samples is used for the diagnosis of canine pheochromocytoma (PC). We describe a simple analytical method based on liquid chromatography tandem mass spectrometry ( LC- MS/MS) for measuring free metanephrine (MN) and normetanephrine (NMN) in spot urine samples. Using the developed method, we evaluated the stability of urinary free-MN and free-NMN at various storing conditions. In addition, we assessed the feasibility of urinary free-MN and -NMN measurement for diagnosing PC. Urine samples were mixed with stable isotope internal standards and thereafter purified by ultrafiltration. The purified samples were analyzed by LC-MS/MS in the multiple reaction monitoring mode after separation on a multimode octa decyl silyl column. The coefficient of variation of free-MN and -NMN measurement was 7.6% and 5.5%, respectively. The linearity range was 0.5-10 mu g/l for both analytes. Degradation was less than 10% for both analytes under any of the storage conditions. The median free-NMN ratio to creatinine of 9 PC dogs (595, range 144-47,961) was significantly higher (P< 0.05) than that of 13 dogs with hypercortisolism (125, range 52-224) or 15 healthy dogs (85, range 50-117). The developed method is simple and may not require acidification of spot urine. The results of this preliminary retrospective study suggest that the measurement of urinary free metanephrines is a promising tool for diagnosing canine PC. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/82669 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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