| Title: | Analysis of serum lysophosphatidylethanolamine levels in patients with non-alcoholic fatty liver disease by liquid chromatography-tandem mass spectrometry |
| Authors: | Yamamoto, Yusuke Browse this author |
| Sakurai, Toshihiro Browse this author |
| Chen, Zhen Browse this author →KAKEN DB |
| Furukawa, Takayuki Browse this author →KAKEN DB |
| Gowda, Siddabasave Gowda B. Browse this author |
| Wu, Yue Browse this author |
| Nouso, Kazuhiro Browse this author →KAKEN DB |
| Fujii, Yuki Browse this author |
| Yoshikawa, Yuki Browse this author |
| Chiba, Hitoshi Browse this author →KAKEN DB |
| Hui, Shu-Ping Browse this author →KAKEN DB |
| Keywords: | Lysophosphatidylethanolamine |
| LysoPE |
| Liquid chromatography-tandem mass spectrometry |
| Chemical synthesis |
| Non-alcoholic fatty liver disease |
| Issue Date: | 22-Oct-2020 |
| Publisher: | Springer |
| Journal Title: | Analytical and bioanalytical chemistry |
| Volume: | 413 |
| Issue: | 1 |
| Start Page: | 245 |
| End Page: | 254 |
| Publisher DOI: | 10.1007/s00216-020-02996-9 |
| PMID: | 33090255 |
| Abstract: | Lysophosphatidylethanolamines (LysoPEs) are the partial hydrolysis products of phosphatidylethanolamine. Despite the unique in vitro bioactivities of LysoPEs, there are limited reports on the pathophysiological role of LysoPEs in the serum, due to the lack of sensitive analytical methods for determination of each molecular species in clinical samples. Herein, we developed a highly sensitive quantitative method to profile the serum LysoPE species by liquid chromatography-tandem mass spectrometry (LC-MS/MS) with selected reaction monitoring (SRM). The internal standard (IS), chemically synthesized in-house, and the lineup of seven major LysoPE species were used in this study. The limits of detection and quantification for each LysoPE species ranged within 0.5-3.3 pmol/mL and 1.0-5.0 pmol/mL, respectively. The combined concentrations of LysoPEs in the serum from healthy subjects (n = 8) and the patients with non-alcoholic fatty liver diseases (NAFLD) including simple steatosis (SS, n = 9) and non-alcoholic steatohepatitis (NASH, n = 27) were 18.030 +/- 3.832, 4.867 +/- 1.852, and 5.497 +/- 2.495 nmol/mL, respectively. The combined and individual concentrations of LysoPEs, except for LysoPE 18:0, significantly decreased in the patients with NAFLD compared with those for the healthy subjects. However, no significant difference was observed between the SS and NASH groups. Our proposed LC-MS/MS method is valid and has advantages of small sample volume, high sensitivity, and simultaneous absolute quantitation for multiple molecular species. This method may enable diagnostic evaluation and elucidation of the as-yet uncovered pathophysiological role of LysoPEs. |
| Rights: | This is a post-peer-review, pre-copyedit version of an article published in Analytical and bioanalytical chemistry. The final authenticated version is available online at: http://dx.doi.org/10.1007/s00216-020-02996-9. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/83041 |
| Appears in Collections: | 保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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