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Up-regulation of P450arom and down-regulation of Dmrt-1 genes in the temperaturedependent sex reversal from genetic males to phenotypic females in a salamander
| Title: | Up-regulation of P450arom and down-regulation of Dmrt-1 genes in the temperaturedependent sex reversal from genetic males to phenotypic females in a salamander |
| Authors: | Sakata, Natsuko Browse this author | | Miyazaki, Kinuko Browse this author | | Wakahara, Masami Browse this author |
| Keywords: | Dmrt-1 | | P450arom | | salamander | | sex differentiation | | sex reversal |
| Issue Date: | Apr-2006 |
| Publisher: | Springer |
| Journal Title: | Development Genes and Evolution |
| Volume: | 216 |
| Issue: | 4 |
| Start Page: | 224 |
| End Page: | 228 |
| Publisher DOI: | 10.1007/s00427-005-0053-4 |
| PMID: | 16506070 |
| Abstract: | When larvae of the salamander Hynobius retardatus were reared at a high temperature (28°C) during their thermosensitive period (TSP: from 15 to 30 days after hatching), all larvae developed to phenotypic females irrespective of their genetic sexes. Hynobius P450 aromatase (P450arom) and Dmrt-1 cDNAs were isolated and their expression pattern was analyzed by competitive and conventional RT-PCR. While P450arom gene was expressed predominantly in the ovary, Dmrt-1 was expressed exclusively in the testis. When larvae were reared at the female-producing temperature (28°C) during the TSP, strong expression of the P450arom and complete suppression of the Dmrt-1 genes were induced in all experimental larvae. Up-regulation of the P450arom and down-regulation of Dmrt-1 genes even in genetic males constitute a part of the molecular biological cascade for the temperature-dependent sex reversal from genetic males to phenotypic females in this salamander. |
| Description: | The original publication is available at http://www.springerlink.com/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/8401 |
| Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 若原 正己
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