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Metabolomic Alteration in the Plasma of Wild Rodents Environmentally Exposed to Lead : A Preliminary Study
| Title: | Metabolomic Alteration in the Plasma of Wild Rodents Environmentally Exposed to Lead : A Preliminary Study |
| Authors: | Nakata, Hokuto Browse this author →KAKEN DB | | Eguchi, Akifumi Browse this author | | Nakayama, Shouta M. M. Browse this author →KAKEN DB | | Yabe, John Browse this author | | Muzandu, Kaampwe Browse this author | | Ikenaka, Yoshinori Browse this author →KAKEN DB | | Mori, Chisato Browse this author | | Ishizuka, Mayumi Browse this author →KAKEN DB |
| Keywords: | lead | | metabolomics | | wild rodent | | mining | | biomarker | | biological pathway | | lasso regression model | | random forest model |
| Issue Date: | Jan-2022 |
| Publisher: | MDPI |
| Journal Title: | International journal of environmental research and public health |
| Volume: | 19 |
| Issue: | 1 |
| Start Page: | 541 |
| Publisher DOI: | 10.3390/ijerph19010541 |
| Abstract: | Lead poisoning is often considered a traditional disease; however, the specific mechanism of toxicity remains unclear. The study of Pb-induced alterations in cellular metabolic pathways is important to understand the biological response and disorders associated with environmental exposure to lead. Metabolomics studies have recently been paid considerable attention to understand in detail the biological response to lead exposure and the associated toxicity mechanisms. In the present study, wild rodents collected from an area contaminated with lead (N = 18) and a control area (N = 10) were investigated. This was the first ever experimental metabolomic study of wildlife exposed to lead in the field. While the levels of plasma phenylalanine and isoleucine were significantly higher in a lead-contaminated area versus the control area, hydroxybutyric acid was marginally significantly higher in the contaminated area, suggesting the possibility of enhancement of lipid metabolism. In the interregional least-absolute shrinkage and selection operator (lasso) regression model analysis, phenylalanine and isoleucine were identified as possible biomarkers, which is in agreement with the random forest model. In addition, in the random forest model, glutaric acid, glutamine, and hydroxybutyric acid were selected. In agreement with previous studies, enrichment analysis showed alterations in the urea cycle and ATP-binding cassette transporter pathways. Although regional rodent species bias was observed in this study, and the relatively small sample size should be taken into account, the present results are to some extent consistent with those of previous studies on humans and laboratory animals. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/84737 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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