| Title: | Genetic Diversity and Transmission of Multidrug-Resistant Mycobacterium tuberculosis strains in Lusaka, Zambia |
| Authors: | Chizimu, Joseph Yamweka Browse this author |
| Solo, Eddie Samuneti Browse this author |
| Bwalya, Precious Browse this author |
| Kapalamula, Thoko Flav Browse this author |
| Akapelwa, Mwangala Lonah Browse this author |
| Lungu, Patrick Browse this author |
| Shrestha, Dipti Browse this author |
| Fukushima, Yukari Browse this author |
| Mukonka, Victor Browse this author |
| Thapa, Jeewan Browse this author |
| Nakajima, Chie Browse this author |
| Suzuki, Yasuhiko Browse this author →KAKEN DB |
| Keywords: | Mycobacterium tuberculosis |
| multidrug resistance |
| mycobacterial interspersed repetitive |
| units-variable number of tandem repeats (MIRU-VNTR) |
| Zambia |
| Issue Date: | 1-Jan-2022 |
| Publisher: | Elsevier |
| Journal Title: | International journal of infectious diseases |
| Volume: | 114 |
| Start Page: | 142 |
| End Page: | 150 |
| Publisher DOI: | 10.1016/j.ijid.2021.10.044 |
| Abstract: | Objective: Zambia is among the 30 high tuberculosis burden countries in the world. Despite increasing reports of multidrug-resistant tuberculosis (MDR-TB) in routine surveillance, information on the transmission of MDR Mycobacterium tuberculosis strains is largely unknown. This study elucidated the genetic diversity and transmission of MDR M. tuberculosis strains in Lusaka, Zambia. Methods: Eighty-five MDR M. tuberculosis samples collected from 2013 to 2017 at the University Teaching Hospital were used. Drug-resistance associated gene sequencing, spoligotyping, 24-loci mycobacterial interspersed repetitive units-variable number of tandem repeats (MIRU-VNTR), and multiplex PCR for RD Rio sub-lineage identification were applied. Results: The identified clades were LAM (48%), CAS (29%), T (14%), X (6%) and Harlem (2%). Strains belonging to SITs 21/CAS1-Kili and 20/LAM1 formed the largest clonal complexes. Combined spoligotyping and 24 loci-MIRU-VNTR revealed 47 genotypic patterns with a clustering rate of 63%. Ninety-five percent of LAM strains belonged to the RD-Rio sub-lineage. Conclusion: The high clustering rate suggested that a large proportion of MDR-TB was due to recent transmission rather than the independent acquisition of MDR. This spread was attributed to clonal expansion of SIT21/CAS1-Kili and SIT20/LAM1 strains. Therefore, TB control programs recommending genotyping coupled with conventional epidemiological methods can guide measures for stopping the spread of MDR-TB. (C) 2021 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/86361 |
| Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
