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Antiviral Effects of 5-Aminolevulinic Acid Phosphate against Classical Swine Fever Virus : In Vitro and In Vivo Evaluation
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| Title: | Antiviral Effects of 5-Aminolevulinic Acid Phosphate against Classical Swine Fever Virus : In Vitro and In Vivo Evaluation |
| Authors: | Hirose, Shizuka Browse this author | | Isoda, Norikazu Browse this author →KAKEN DB | | Huynh, Loc Tan Browse this author | | Kim, Taksoo Browse this author | | Yoshimoto, Keiichiro Browse this author | | Tanaka, Tohru Browse this author | | Inui, Kenjiro Browse this author | | Hiono, Takahiro Browse this author →KAKEN DB | | Sakoda, Yoshihiro Browse this author →KAKEN DB |
| Keywords: | 5-aminolevulinic acid phosphate | | antiviral effect | | classical swine fever virus | | protoporphyrin IX |
| Issue Date: | 27-Jan-2022 |
| Publisher: | MDPI |
| Journal Title: | Pathogens |
| Volume: | 11 |
| Issue: | 2 |
| Start Page: | 164 |
| Publisher DOI: | 10.3390/pathogens11020164 |
| Abstract: | The inhibitory effects of 5-aminolevulinic acid phosphate (5-ALA), an important amino acid for energy production in the host, against viral infections were previously reported. Here, the antiviral effects of 5-ALA against classical swine fever virus (CSFV) belonging to the genus Pestivirus in the Flaviviridae family and its possible mechanisms were investigated. CSFV replication was suppressed in swine cells supplemented with 5-ALA or its metabolite, protoporphyrin IX (PPIX). The infectivity titer of CSFV was decreased after mixing with PPIX extracellularly. In addition, the activities of the replication cycle were decreased in the presence of PPIX based on the CSFV replicon assay. These results showed that PPIX exerted antiviral effects by inactivating virus particles and inhibiting the replication cycle. To evaluate the in vivo efficacy of 5-ALA, pigs were supplemented daily with 5-ALA for 1 week before virus inoculation and then inoculated with a virulent CSFV strain at the 10(7.0) 50% tissue culture infectious dose. The clinical scores of the supplemented group were significantly lower than those of the nonsupplemented group, whereas the virus growth was not. Taken together, 5-ALA showed antiviral effects against CSFV in vitro, and PPIX played a key role by inactivating virus particles extracellularly and inhibiting the replication cycle intracellularly. |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/86514 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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