2024-03-29T02:05:19Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/113872022-11-17T02:08:08Zhdl_2115_20048hdl_2115_140Effects of Ceramide Inhibition on Radiation-induced Apoptosis in Human Leukemia MOLT-4 CellsTAKAHASHI, Eriko1000010193559INANAMI, OsamuASANUMA, TaketoshiKUWABARA, Mikinoriopen accessCeramideFumonisin B1MOLT-4SphingomyelinaseX irradiation490In the present study, using inhibitors of ceramide synthase (fumonisin B1), ketosphinganine synthetase (L-cycloserine), acid sphingomyelinase (D609 and desipramine) and neutral sphingomyelinase (GW4869), the role of ceramide in X-ray-induced apoptosis was investigated in MOLT-4 cells. The diacylglycerol kinase (DGK) assay showed that the intracellular concentration of ceramide increased time-dependently after X irradiation of cells, and this radiation-induced accumulation of ceramide did not occur prior to the appearance of apoptotic cells. Treatment with D609 significantly inhibited radiation-induced apoptosis, but did not inhibit the increase of intracellular ceramide. Treatment with desipramine or GW4869 prevented neither radiation-induced apoptosis nor the induced increase of ceramide. On the other hand, fumonisin B1 and L-cycloserine had no effect on the radiation-induced induction of apoptosis, in spite of significant inhibition of the radiation-induced ceramide. From these results, it was suggested that the increase of the intracellular concentration of ceramide was not essential for radiation-induced apoptosis in MOLT-4 cells.日本放射線影響学会2006-03-30engjournal articleVoRhttp://hdl.handle.net/2115/11387https://doi.org/10.1269/jrr.47.190449-30601349-9157Journal of Radiation Research4711925https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/11387/1/JRR47_19.pdfapplication/pdf331.49 KB2006-03-30