2024-03-29T12:08:12Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/287802022-11-17T02:08:08Zhdl_2115_20044hdl_2115_124LIF- and IL-6-Induced Acetylation of STAT3 at Lys-685 through PI3K/Akt Activation.Ohbayashi, NorihikoIkeda, OsamuTaira, NaohisaYamamoto, YuMuromoto, RyutaSekine, YuichiSugiyama, KenjiHonjoh, Tsutomu1000020212219Matsuda, Tadashiopen accesssignal transducer and activator of transcription 3interleukin-6acetylationleukemia inhibitory factorPI3KAkt499.3Signal transducer and activator of transcription 3 (STAT3), which mediates biological actions in many physiological processes, is activated by cytokines and growth factors via specific tyrosine or serine phosphorylation, dimerization and nuclear translocation. A recent study has demonstrated, by using antibody to acetylated lysine, and a STAT3 mutant with Lys-685-to-Arg substitution, that STAT3 is acetylated at Lys-685 by histone acetyltransferase p300, and that acetylation at Lys-685 is critical for STAT3 activation. In the present study, we created an acetyl-specific antibody against STAT3 acetylated at Lys-685, and found that leukemia inhibitory factor (LIF) or interleukin (IL)-6 induced acetylation of STAT3 at Lys-685 in 293T and Hep3B cells. Moreover, acetylation of STAT3 at Lys-685 was suppressed by PI3K inhibitor LY294002, or a dominant negative Akt. Taken together, our findings demonstrate that endogenous STAT3 is acetylated at Lys-685 by LIF or IL-6 through PI3K/Akt activation.日本薬学会The Pharmaceutical Society of Japan2007-10engjournal articleVoRhttp://hdl.handle.net/2115/28780https://doi.org/10.1248/bpb.30.1860179172510918-61581347-5215Biological & Pharmaceutical Bulletin301018601864https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/28780/1/BPB30-10.pdfapplication/pdf710.8 KB2007-10