2024-03-28T21:39:29Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/323192022-11-17T02:08:08Zhdl_2115_20048hdl_2115_140Testicular toxicity of profenofos in matured male ratsMoustafa, Gihan GamalIbrahim, Zein Shaban1000020091481Hashimoto, YoshiharuAlkelch, M. Alkelch1000080374627Sakamoto, Kentaro Q.1000050332474Ishizuka, Mayumi1000010143314Fujita, Shoichiopen accessThe original publication is available at www.springerlink.comprofenofoscytochrome P450testosteronetestis649To investigate the effect of the phosphorothoate insecticide profenofos on male specific gene expression on rat testis, 16-week-old Wistar rats were orally administered at dose of 17.8 mg/kg twice weekly for 65 days. Gene expression in the testes was monitored by DNA microarray analysis and real-time RT-PCR, which revealed that genes related to steroidogenesis including cytochrome P450 17A1 (CYP17A1), steroidogenic acute regulatory protein (StAR) and CYP11A1 were significantly increased. Besides the testes were histopathologicaly examined, which revealed testicular destruction and degeneration represented by a layer of columnar epithelium, oedematous changes surrounding the seminiferous tubules besides vacuolated spermatogonial cells and more elongated Leydig cells. These data suggest that profenofos considered as one of the male reproductive toxicants. Furthermore, we propose that the above three steroidogenic-related genes and the gene of acrosomal reaction as potential biomarkers of testicular toxicity.Springer2007-12engjournal articleAMhttp://hdl.handle.net/2115/32319http://www.springerlink.comhttps://doi.org/10.1007/s00204-007-0217-2175690320340-57611432-0738Archives of Toxicology8112875881https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/32319/1/AOT81-12.pdfapplication/pdf837.3 KB2007-12