2024-03-29T04:33:51Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/386302022-11-17T02:08:08Zhdl_2115_20044hdl_2115_124Incorporation of 8-hydroxyguanosine (8-oxo-7,8-dihydroguanosine) 5'-triphosphate by bacterial and human RNA polymerasesIncorporation of 8-hydroxyguanosine 5'-triphosphate (8-oxo-7,8-dihydroguanosine 5'-triphosphate) by bacterial and human RNA polymerasesKamiya, HiroyukiSuzuki, AkihiroYamaguchi, YukiHanda, Hiroshi1000000183567Harashima, Hideyoshiopen accessOxidized ribonucleotide8-Hydroxyguanosine 5'-triphosphateRNA polymeraseTranscriptionFree radicals499Oxidized RNA precursors formed in the nucleotide pool may be incorporated into RNA. In this study, the incorporation of 8-hydroxyguanosine 5'-triphosphate (8-OH-GTP; 8-oxo-7,8-dihydroguanosine 5'-triphosphate) into RNA by Escherichia coli RNA polymerase was examined in vitro, using a primer RNA and a template DNA with defined sequences. 8-OH-GTP was incorporated opposite C and A in the template DNA. Surprisingly, 8-OH-GTP was quite efficiently incorporated by the bacterial RNA polymerase, in contrast to the incorporation of the 2'-deoxyribo-counterpart by DNA polymerases, as indicated by the kinetic parameters. The primer was further extended by the addition of a ribonucleotide complementary to the nucleobase adjacent to C or A (the nucleobase opposite which 8-OH-GTP was inserted). Thus, the incorporation of 8-OH-GTP did not completely inhibit further RNA chain elongation. 8-OH-GTP was also incorporated opposite C and A by human RNA polymerase II. These results suggest that 8-OH-GTP in the nucleotide pool call cause the formation of oxidized RNA and disturb the transmittance of genetic information.Elsevier Inc.2009-06-15engjournal articleAMhttp://hdl.handle.net/2115/38630https://doi.org/10.1016/j.freeradbiomed.2009.04.005193621410891-5849Free Radical Biology and Medicine461217031707https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/38630/1/46-12_p1703-1707.pdfapplication/pdf490.4 KB2009-06-15