2024-03-29T15:48:41Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/393922022-11-17T02:08:08Zhdl_2115_20049hdl_2115_141Simultaneous formation of haploid, diploid and triploid eggs in diploid-triploid mosaic loaches1N, 2N and 3N eggs of mosaic loachesYoshikawa, H.Morishima, K.Fujimoto, T.Yamaha, E.1000000137902Arai, K.open accessThe definitive version is available at www.blackwellsynergy.comMisgurnus anguillicaudatusmosaicclonepolyploid666In the loach Misgurnus anguillicaudatus, very few diploid-triploid mosaic individuals, which are generated by accidental incorporation of the sperm nucleus into diploid eggs produced by clonal diploid loach, occur in nature. Ploidy examination of gynogenetic progeny induced by activation with UV-irradiated goldfish sperm indicated that diploid-triploid mosaic females laid haploid, diploid and triploid eggs, simultaneously. In addition, triploid eggs exhibited larger egg sizes. Microsatellite genotyping of diploid-triploid mosaics revealed that triploid genotypes of mosaic mothers consisted of two alleles specific to the clonal diploid and one allele from normal diploid male. Diploid eggs from mosaic mother had genotypes absolutely identical to the diploid clone. Most genotypes of triploid eggs were identical to the mosaic mother, and one of three alleles of the mosaic mother was transmitted to haploid eggs. These results suggested that diploid germ cells, which had a clonal genome, were differentiated into clonal diploid eggs, and triploid and haploid eggs were produced from triploid germ cells in the same ovary of mosaic individuals.Fisheries Society of the British Isles2007-10engjournal articleAMhttp://hdl.handle.net/2115/39392https://doi.org/10.1111/j.1095-8649.2007.01608.x0022-11121095-8649AA00698121Journal of Fish Biology71sb250263https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/39392/1/arai-119.pdfapplication/pdf397.89 KB2007-10