2024-03-29T06:34:08Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/441582022-11-17T02:08:08Zhdl_2115_20055hdl_2115_8527Synthesis of the glycosaminoglycan-protein linkage tetraosyl peptide moieties of betaglycan, which serve as a hexosamine acceptor for enzymatic glycosyl transferTamura, Jun-ichiNakamura-Yamamoto, TomomiNishimura, YukoMizumoto, ShujiTakahashi, JunSugahara, Kazuyukiopen accessChondroitin sulfateHeparan sulfateGlycoconjugate synthesisGlycosyl transferN-Acetylgalactosaminyltransferase-Iα1,4-N-Acetylglucosaminyltransferase-I464Betaglycan, also known as TGF-β type III receptor, is a membrane-anchored proteoglycan, which has two glycosaminoglycan (GAG) attachment sites (López-Casillas et.al. J. Cell Biol. 1994, 124, 557-568). Chondroitin sulfate (CS) or heparan sulfate (HS) can attach to the first site, Ser535, whereas only CS attaches to the second, Ser546. Although the mechanism behind the assembly of CS and HS is not fully understood, it has been reported that the assembly of HS requires not only a cluster of acidic residues but also hydrophobic residues located near the Ser-Gly attachment sites (Esko, J. D. Zhang, L Curr. Opin. Struct. Biol. 1996, 6, 663-670). To further understand the effects of amino acids close to the Ser residues of the GAG-attachment sites on the glycosyltransferases, two tetraosyl peptides derived from the CS attachment sites of betaglycan, GLcA-Gal-Gal-Xyl-SerGlyAspAsnGly (1) and GLcA-Gal-Gal-Xyl-SerGlyAspAsnGlyPheProGly (2), were synthesized, and used as donor substrates for β1,4-N-acetylgalactosaminyltransferase-I (β4GaINAcT-I) and α1,4-N-acetylglucosaminyltransferase-I (α4GlcNAcT-I). Both the chemically synthesized linkage region tetrasaccharides were far better acceptors for β4GalNAcT-I than for α4GlcNAcT-1 in vitro, although they also showed appreciable acceptor activity for α4GlcNAcT-I.Elsevier2010-10-13engjournal articleAMhttp://hdl.handle.net/2115/44158https://doi.org/10.1016/j.carres.2010.06.0190008-6215Carbohydrate Research3451521152123https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/44158/1/CR345-15_2115-2123.pdfapplication/pdf448.63 KB2010-10-13