2024-03-29T09:30:29Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/484882022-11-17T02:08:08Zhdl_2115_20046hdl_2115_138Phylogenetic diversity and dietary association of rumen Treponema revealed using group-specific 16S rRNA gene-based analysisBekele, Aschalew Z.1000090431353Koike, SatoshiKobayashi, Yasuoopen accessThe definitive version is available at www.blackwell-synergy.comrumenTreponemadiversitydiet association16S rRNA gene465Treponema spp. are a commonly detected bacterial group in the rumen that are involved in the degradation of soluble fibers. In this study, a ruminal Treponema group-specific PCR primer targeting the 16S rRNA gene was designed and used to assess the phylogenetic diversity and diet association of this group in sheep rumen. Total DNA was extracted from rumen digesta of three sheep fed a diet based on alfalfa/orchardgrass hay or concentrate. The real-time PCR quantification indicated that the relative abundance of the Treponema group in the total rumen bacteria was as high as 1.05%, while the known species Treponema bryantii accounted for only 0.02%. Fingerprints of the Treponema community determined by 16S rDNA-targeted denaturing gradient gel electrophoresis (DGGE) analysis tended to differ among the diets. Principal component analysis of the DGGE profiles distinguished those Treponema associated with either the hay or the concentrate diets. Analysis of a Treponema 16S rRNA gene clone library showed phylogenetically distinct operational taxonomic units for a specific dietary condition, and significant (P=0.001) differences in community composition were observed among clone libraries constructed from each dietary regimen. The majority of clones (75.4%) had less than 97% sequence similarity with known Treponema. These results suggest the predominance of uncultured Treponema that appear to have distinct members related to the digestion of either hay or concentrate diet.Blackwell Publishing2011-03engjournal articleAMhttp://hdl.handle.net/2115/48488https://doi.org/10.1111/j.1574-6968.2010.02191.x212049270378-1097FEMS Microbiology Letters31615160https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/48488/1/FEMSML316-1_51-60.pdfapplication/pdf826.06 KB2011-03