2024-03-28T22:32:02Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/486422022-11-17T02:08:08Zhdl_2115_20046hdl_2115_138An intact mitochondrial cox1 gene and a pseudogene with different genomic configurations are present in apple cultivars 'Golden Delicious' and 'Delicious': Evolutionary aspectsWakatsuki, AkihitoKitazaki, KazuyoshiKato, Sumie1000060192556Kishima, YujiMikami, Tetsuoopen access467We have characterized the mitochondrial cox1 gene copies in two apple cultivars 'Golden Delicious' and 'Delicious'. Both the cultivars contained an intact copy and a truncated copy of cox1. The intact 'Golden Delicious' and 'Delicious' cox1 genes, designated G-cox1 and D-cox1, respectively, were both found to be actually transcribed to give an RNA of approximately 1.7 kb. The two intact cox1 and two truncated copies (G-φcox1 and D-φcox1) shared a common 1115-bp segment flanked by four combinations of two different 5'- and 3'-sequences. PCR assay demonstrated that the configurations bearing G-cox1 and G-φcox1 existed in substoichiometric amounts within the mitochondrial genome of 'Delicious' whereas substoichiometric molecules carrying D-φcox1 were present in the 'Golden Delicious' mitochondrial genome. Although ancestor/descendant relationships cannot be inferred between the G-cox1 and D-cox1 arrangements, the results led us to hypothesize that (1) the 1115-bp segment containing part of the progenitor cox1 was duplicated, thereby generating a pseudo-cox1 copy, and (2) this was followed by homologous recombination across a portion of the 1115-bp repeats which gave rise to the descendant cox1 and pseudo-cox1 arrangements.Elsevier B.V.2011-08-26engjournal articleAMhttp://hdl.handle.net/2115/48642https://doi.org/10.1016/j.scienta.2011.05.0370304-4238Scientia Horticulturae13014953https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/48642/1/SH130-1_49-53.pdfapplication/pdf1.2 MB2011-08-26