2024-03-28T13:58:54Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/503912022-11-17T02:08:08Zhdl_2115_20044hdl_2115_124Transport of fluorescent chenodeoxycholic acid via the human organic anion transporters OATP1B1 and OATP1B3.1000080400373Yamaguchi, HiroakiOkada, MasahiroAkitaya, ShouOhara, HiroshiMikkaichi, TsuyoshiIshikawa, HarunaSato, MayumiMatsuura, MasakiSaga, ToshihideUnno, MichiakiAbe, TakaakiMano, NariyasuHishinuma, TakanoriGoto, Junichiopen accessnitrobenz-2-oxa-1,3-diazoleorganic anion-transporting polypeptide 1B1organic anion-transporting polypeptide 1B3visualizationBile Acids and Salts/chemistryBile Acids and Salts/metabolismBile Acids and Salts/pharmacokineticsBiological TransportCell Line, TumorChenodeoxycholic Acid/chemistryChenodeoxycholic Acid/metabolismChenodeoxycholic Acid/pharmacokineticsHumansMicroscopy, ConfocalMolecular StructureNitrobenzenes/chemistryNitrobenzenes/metabolismNitrobenzenes/pharmacokineticsOrganic Anion Transport Protein 1/antagonists & inhibitorsOrganic Anion Transport Protein 1/geneticsOrganic Anion Transport Protein 1/metabolismOrganic Anion Transporters, Sodium-Independent/geneticsOrganic Anion Transporters, Sodium-Independent/metabolismOxazoles/chemistryOxazoles/metabolismOxazoles/pharmacokineticsTime Factors499This study sought to clarify the contributions of organic anion-transporting polypeptide (OATP) 1B1 and 1B3 to the liver uptake of chenodeoxycholic acid (CDCA). We synthesized a fluorescent version of CDCA, chenodeoxychilyl-(Nepsilon-NBD)-lysine (CDCA-NBD), to characterize transporter-mediated uptake. CDCA-NBD is efficiently transported by OATP1B1 and OATP1B3 with high affinities. The Michaelis-Menten constants for CDCA-NBD uptake by OATP1B1 and OATP1B3 were 1.45 +/- 0.39 microM and 0.54 +/- 0.09 microM, respectively. By confocal laser scanning microscopy, CDCA-NBD, which is taken up by OATP1B1 and OATP1B3, was observed to localize to the cytosol. We also examined the transport of newly synthesized fluorescent bile acids. NBD-labeled bile acids, including cholic acid, deoxycholic acid, lithocholic acid, and ursodeoxycholic acid, were all transported by OATP1B1 and OATP1B3. CDCA-NBD exhibited the highest rate of transport of the five NBD-labeled bile acids examined in OATP1B1- and OATP1B3-expressing cells. Our results suggest that OATP1B1 and OATP1B3 play important roles in CDCA uptake into the liver. Fluorescent bile acids are useful tools to characterize the uptake properties of membrane transporters.American Society for Biochemistry and Molecular Biology2006-06engjournal articleAMhttp://hdl.handle.net/2115/50391https://doi.org/10.1194/jlr.M500532-JLR200165341400022-22751539-7262AA00701215Journal of lipid research47611961202https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/50391/3/06_20121105.pdfapplication/pdf194.37 KB2006-06