2024-03-29T05:22:35Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/507822022-11-17T02:08:08Zhdl_2115_20076hdl_2115_597A New Loop-Mediated Isothermal Amplification Method for Rapid, Simple, and Sensitive Detection of Leptospira spp. in UrineKoizumi, Nobuo1000060435964Nakajima, ChieHarunari, TsunehitoTanikawa, TsutomuTokiwa, ToshihiroUchimura, ErikoFuruya, TokujiroMingala, Claro NiegosVillanueva, Marvin ArdezaOhnishi, Makoto1000090206540Suzuki, Yasuhikoopen access© 2012 American Society for Microbiology465We developed a new loop-mediated isothermal amplification (LAMP) method to detect rrs, a 16S rRNA gene, of pathogenic Leptospira spp. in urine. The method enables detection of two leptospiral cells per reaction following boiling of urine specimens. The sensitivity of this method is higher than that of culture or of flaB-nested PCR.American Society for Microbiology2012-06engjournal articleAMhttp://hdl.handle.net/2115/50782https://doi.org/10.1128/JCM.00481-120095-1137Journal of Clinical Microbiology50620722074https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/50782/2/JCM50-6_2072-2074.pdfapplication/pdf222.08 KB2012-06https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/50782/1/Supplemental_material.pdfapplication/pdf322.88 KB2012-06