2024-03-29T15:33:49Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/527312022-11-17T02:08:08Zhdl_2115_52005hdl_2115_52004Effectiveness of xylose utilization for high yield production of lactate-enriched P(lactate-co-3-hydroxybutyrate) using a lactate-overproducing strain of Escherichia coli and an evolved lactate-polymerizing enzymeNduko, John Masani1000080360642Matsumoto, Ken'ichiro1000040223713Ooi, Toshihiko1000070216828Taguchi, Seiichiopen accessLignocelluloseBio-based plasticPolylactic acidMicrobial cell factory464Xylose, which is a major constituent of lignocellulosic biomass, was utilized for the production of poly(lactate-co-3-hydroxybutyrate) [P(LA-co-3HB)], having transparent and flexible properties. The recombinant Escherichia coil JW0885 (pflA(-)) expressing LA-polymerizing enzyme (LPE) and monomer supplying enzymes grown on xylose produced a copolymer having a higher LA fraction (34 mol%) than that grown on glucose (26 mol%). This benefit of xylose was further enhanced by combining it with an evolved LPE (ST/FS/QK), achieving a copolymer production having 60 mol% LA from xylose, while glucose gave a 47 mol% LA under the same condition. The overall carbon yields from the sugars to the polymer were similar for xylose and glucose, but the ratio of the LA and 3HB units in the copolymer was different. Notably, the P(LA-co-3HB) yield from xylose (7.3 g l(-1)) was remarkably higher than that of P(3HB) (4.1 g l(-1)), indicating P(LA-co-3HB) as a potent target for xylose utilization. (C) 2012 Elsevier Inc. All rights reserved.ELSEVIER2013-01engjournal articleAMhttp://hdl.handle.net/2115/52731https://doi.org/10.1016/j.ymben.2012.11.007232027501096-7176METABOLIC ENGINEERING15159166https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/52731/1/Metabolic%20engineering%20submitted%20file-11.pdfapplication/pdf809.21 KB2013-01