2024-03-28T09:37:04Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/531322022-11-17T02:08:08Zhdl_2115_20045hdl_2115_139Determination of Labile Fe(II) Species Complexed with Seawater Extractable Organic Matter Under Seawater Conditions Based on the Kinetics of Ligand-exchange Reactions with FerrozineIwai, Hisanori1000040344113Fukushima, MasamiYamamoto, Mitsuoopen accessSeawater extractable organic matterironlabilitycomplexferrozineseawaterstability constantbinding capacity431A fertilizer, comprised of a mixture of steel slag and compost, was used to restore seaweed beds in barren coastal areas. Complex Fe(I) species, supplied by steel slag, play a significant role in supplying Fe(II) to coastal areas and stimulating seaweed growth. Seawater extractable organic matter (SWEOM) from compost is generally assumed to serve as a chelator of Fe(II) in the fertilizer. It is considered that the bioavailability of Fe(II)-SWEOM complexes is higher in the dissociable (labile) species. In the present study, a method for determining labile species of Fe(II)-SWEOM complexes in seawater (pH 8.0, I = 0.7) was developed. The method is based on a ligand-exchange reaction between SWEOM and ferrozine (FZ). Because Fe(II) is readily oxidized to Fe(III) under normal seawater conditions, ascorbic acid was added as an antioxidant. The coloring for the Fe-FZ complex in the presence of SWEOM was retarded. This retarding can be attributed to a ligand-exchange reaction between FZ and labile Fe(II)-SWEOM complexes. Conditional binding constants for the labile Fe(II)-SWEOM complexes and binding capacities of labile sites in SWEOM to Fe(II) were evaluated for a variety of total Fe(II) concentrations.Japan Soc Analytical Chemistry2013-07engjournal articleVoRhttp://hdl.handle.net/2115/53132https://doi.org/10.2116/analsci.29.7230910-6340AA10500785Analytical Sciences297723728https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/53132/1/29_723.pdfapplication/pdf651.67 KB2013-07