2024-03-29T08:14:36Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/539712023-01-19T03:10:04Zhdl_2115_20044hdl_2115_124c-Myc gene single-strand binding protein-1, MSSP-1, suppresses transcription of alpha-smooth muscle actin gene in chicken visceral smooth muscle cells.Kimura, KazuhiroSaga, Hiroshi1000090238105Hayashi, Ken'ichiroObata, HidetoChimori, Yoshihiro1000020143505Ariga, Hiroyoshi1000020112047Sobue, Kenjiopen accessActins/geneticsAnimalsCell ExtractsCell NucleusCells, CulturedChick EmbryoCloning, MolecularDNA/metabolismDNA-Binding Proteins/metabolismGene Expression Regulation/geneticsGizzardMolecular Sequence DataMuscle, Smooth/cytologyMuscle, Smooth/metabolismMutationPromoter Regions, Genetic/geneticsRNA-Binding ProteinsRecombinant Fusion ProteinsTranscription, Genetic/genetics499The expression of alpha-smooth muscle actin is coordinately regulated by positive and negative cis- elements in the promoter region. Although cis -elements and trans -acting factors involved in the positive regulation of the alpha-smooth muscle (alpha-SM) actin gene have been well characterized, details of negative regulation remain unclear. In functional analyses using cultured gizzard smooth muscle cells, we identified a sequence ranging from -238 to -219 in the promoter region as a novel negative element. Mutation and deletion analyses further revealed that a sequence, TATCTTA (-228 to -222), is essential for negative regulation. Gel shift assay and Southwestern blotting indicated that a nuclear protein factor specifically interacts with single- or double-strand DNA including this sequence, and the protein factor displays a highly potent binding to the sense strand DNA. cDNA cloning and gel shift analysis using anti-MSSP-1 antibodies revealed that this protein factor is a chicken homolog of human MSSP-1 (c- myc gene single-strand binding protein-1). In fact, overexpression of MSSP-1 in cultured smooth muscle cells suppresses the promoter activity. These results suggest a novel function of MSSP-1 regarding the transcriptional regulation of alpha-sm actin gene.Oxford University Press1998-05-15engjournal articleVoRhttp://hdl.handle.net/2115/53971https://doi.org/10.1093/nar/26.10.242095806950305-1048Nucleic acids research261024202425https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/53971/1/89_Ariga_1998_Nucleic_Acids_Res.pdfapplication/pdf153.05 KB1998-05-15