2024-03-28T09:34:49Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/584562022-11-17T02:08:08Zhdl_2115_20039hdl_2115_116Characterization of the human TCAM1P pseudogene and its activation by a potential dual promoter-enhancer: Comparison with a protein-coding mouse orthologueKurihara, Misuzu1000090422005Kimura, Atsushi P.open access(C) 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.Dual promoter-enhancerHumanLong non-coding RNAUnitary pseudogeneTCAM1PTestis460TCAM1P is a unitary pseudogene, which was disabled since the human-mouse divergence. Here we found that TCAM1P was specifically expressed in the human testis, with different cell type-specificity from mouse Tcam1, and characterized its transcripts. At the mouse locus, a multifunctional dual promoter-enhancer (DPE) controls the expression of Tcam1 and Smarcd2 genes. The corresponding human sequence was found to potentially function as a DPE, although the molecular mechanism was different from mouse. Interestingly, the change in DPE activity occurred before pseudogenization of TCAM1P. These data suggest the presence of a DPE in the human genome for the first time, and provide an important model of evolutionary changes in the regulatory mechanism of a pseudogene.Elsevier2015-02-13engjournal articleAMhttp://hdl.handle.net/2115/58456https://doi.org/10.1016/j.febslet.2015.01.023256228930014-57931873-3468FEBS Letters5894540547https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/58456/1/FEBSLet_589_p540-.pdfapplication/pdf426.18 KB2015-02-13