2024-03-28T11:32:36Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/631962022-11-17T02:08:08Zhdl_2115_20048hdl_2115_140Development of a new device for artificial insemination in cynomolgus macaquesKOYAMA, ShuzoFUKUDA, KojiWATANABE, ShoKOHARA, Sakae1000010378440TSUCHIYA, HideakiFUKUZAKI, Koichiro1000070312402NAGANO, MasashiUNO, Yasuhiro1000070192739HOSOI, Yoshihikoopen accessCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 InternationalArtificial inseminationCynomolgus monkeyDeviceSemen649In cynomolgus macaques, an important animal species for biomedical research, efficient reproduction has been hampered partly due to the difficulties of artificial insemination (AI) using straw tubes developed for humans or farm animals, because cynomolgus macaques have a complex cervical canal structure. In this study, taking into consideration the unique structure of the macaque cervical canal, we developed a novel device for AI, comprised of a syringe and an outer cylinder. At 24 and 48 h after using this device to inject semen into one female, viable sperm were observed in the oviduct where the sperm meets the oocytes. We then attempted AI using this new device on 10 females that were at pre-ovulation, and pregnancy was successful in three animals (30% pregnancy rate). These results show that the newly developed device can be used for AI in cynomolgus macaques.日本繁殖生物学会2016-10engjournal articleVoRhttp://hdl.handle.net/2115/63196https://doi.org/10.1262/jrd.2016-0450916-88181348-4400AA10936678Journal of Reproduction and Development625527529https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/63196/1/Koyama-mfAI-device-16%28JRD%29.pdfapplication/pdf747.46 KB2016-10