2024-03-28T09:14:04Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/681462022-11-17T02:08:08Zhdl_2115_20046hdl_2115_138Macrocarpal C isolated from Eucalyptus globulus inhibits dipeptidyl peptidase 4 in an aggregated form1000040466446Kato, EisukeKawakami, KazuhiroKawabata, Junopen accessThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.Creative Commons Attribution 4.0 InternationalDipeptidyl peptidase 4diabetesEucalyptus globulusmacrocarpal433Dipeptidyl peptidase 4 (DPP-4) inhibitors are used for the treatment of type-2 diabetes mellitus. Various synthetic inhibitors have been developed to date, and plants containing natural DPP-4 inhibitors have also been identified. Here, 13 plant samples were tested for their DPP-4 inhibitory activity. Macrocarpals A-C were isolated from Eucalyptus globulus through activity-guided fractionation and shown to be DPP-4 inhibitors. Of these, macrocarpal C showed the highest inhibitory activity, demonstrating an inhibition curve characterised by a pronounced increase in activity within a narrow concentration range. Evaluation of macrocarpal C solution by turbidity, nuclear magnetic resonance spectroscopy and mass spectrometry indicated its aggregation, which may explain the characteristics of the inhibition curve. These findings will be valuable for further study of potential small molecule DPP-4 inhibitors.Taylor & Francis2017-11-17engjournal articleVoRhttp://hdl.handle.net/2115/68146https://doi.org/10.1080/14756366.2017.13964581475-63661475-6374Journal of enzyme inhibition and medicinal chemistry331106109https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/68146/1/Macrocarpal%20C%20isolated%20from%20Eucalyptus%20globulus%20inhibits%20dipeptidyl%20peptidase%204%20in%20an%20aggregated%20form.pdfapplication/pdf980.62 KB2017-11-17