2024-03-28T23:56:46Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/721342022-11-17T02:08:08Zhdl_2115_20057hdl_2115_148Photoinduced electron transfer reaction in mitochondria for spatiotemporal selective photo-oxidation of lipids by donor/acceptor linked moleculesTakano, YutaHanai, ErinaImahori, Hiroshiopen access425Donor-acceptor-linked molecules have been synthesized and utilized to induce the rapid and site-selective lipid-oxidation in mitochondria by utilizing a photoinduced intermolecular electron transfer reaction. Two water-soluble donor-acceptor molecules (1 and 2) were designed and synthesized for this purpose. 2 was prepared to modulate its affinity to cell membrane in mitochondria. Confocal laser microscopy experiments revealed that 1 and 2 possess high localization abilities in mitochondria. By the photoinduced electron transfer, 2 exhibited the remarkable oxidation ability of lipids, mainly cardiolipin. In HeLa cells, 2 triggered mitochondrial lipid oxidation, which was followed by apoptotic cell death, under illumination within a few seconds. These results show that the present molecular system is highly promising to utilize the photoinduced intermolecular electron transfer reaction in a precise spatiotemporal manner in a cell by using light.Royal Society of Chemistry2017-12-07engjournal articleAMhttp://hdl.handle.net/2115/72134https://doi.org/10.1039/c7nr04919f2040-3364Nanoscale9451790917913https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/72134/1/NanoS170824CSOxCL.pdfapplication/pdf663.74 KB2017-12-07