2024-03-28T22:02:36Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/823912022-11-17T02:08:08Zhdl_2115_20076hdl_2115_597Rapid and Simple Detection of Isoniazid-Resistant Mycobacterium tuberculosis Utilizing a DNA Chromatography-Based TechniqueKodera, TakuyaYamaguchi, TomoyukiFukushima, YukariKobayashi, KumiTakarada, YutakaChizimu, Joseph Yamweka1000060435964Nakajima, ChieSolo, Eddie SamunetiLungu, Patrick SailiKawase, Mitsuo1000090206540Suzuki, Yasuhikometadata only access490Despite the availability of anti-tuberculosis drugs, the treatment of tuberculosis has been complicated by drug-resistant tuberculosis. The early detection of drug resistance makes early treatment possible. However. the available tools are mainly for rifampicin resistance detection, and the existing isoniazid resistance detection method is expensive, highly technical, and complicated, making it unsustainable for use in developing nations. This study aimed to develop a simple, rapid, and low-cost diagnostic kit for isoniazid-resistant tuberculosis using the single-stranded tag hybridization method to target an isoniazid resistance-conferring mutation. Specificity and sensitivity were assessed using DNA extracted from 49 isoniazid-resistant and 41 isoniazid-susceptible Mycobacterium tuberculosis clinical isolates cultured in mycobacterial growth indicator tubes. Positive signals were observed on mutant and wild-type lines with 100% sensitivity and specificity compared with Sanger sequencing results. In contrast, no positive signal was observed for non-tuberculosis mycobacteria. The detection limit of this method was 10(3) CFU or less. The STH-PAS system for isoniazid-resistant M. tuberculosis detection developed in this study offers a better alternative to conventional phenotypic isoniazid resistance determination, which will be of both clinical and epidemiological significance in resource-limited nations.国立感染症研究所(National Institute of Infectious Diseases, Japan)2021-05engjournal articleNAhttp://hdl.handle.net/2115/82391https://doi.org/10.7883/yoken.JJID.2020.7541344-6304Japanese journal of infectious diseases743214219