2024-03-28T18:43:01Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/827822022-11-17T02:08:08Zhdl_2115_20046hdl_2115_138Molecular insight into regioselectivity of transfructosylation catalyzed by GH68 levansucrase and beta-fructofuranosidase1000000344490Okuyama, MasayukiSerizawa, RyoTanuma, MasanariKikuchi, AsakoSadahiro, Juri1000070709849Tagami, TakayoshiLang, Weeranuch1000090186312Kimura, Atsuometadata only accessCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 International400Glycoside hydrolase family 68 (GH68) enzymes catalyze beta-fructosyltransfer from sucrose to another sucrose, the so-called transfructosylation. Although regioselectivity of transfructosylation is divergent in GH68 enzymes, there is insufficient information available on the structural factor(s) involved in the selectivity. Here, we found two GH68 enzymes, beta-fructofuranosidase (FFZm) and levansucrase (LSZm), encoded tandemly in the genome of Zymomonas mobilis, displayed different selectivity: FFZm catalyzed the beta-(2 -> 1)-trans-fructosylation (1-TF), whereas LSZm did both of 1-TF and beta-(2 -> 6)-trans-fructosylation (6-TF). We identified His79(FFZm) and Ala343(FFZm) and their corresponding Asn84(LSZm) and Ser345(LSZm) respectively as the structural factors for those regioselectivities. LSZm with the respective substitution of FFZm-type His and Ala for its Asn84(LSZm) and Ser345(LSZm) (N84H/S345A-LSZm) lost 6-TF and enhanced 1-TF. Conversely, the LSZm-type replacement of His79(FFZm) and Ala343(FFZm) in FFZm (H79N/A343S-FFZm) almost lost 1-TF and acquired 6-TF. H79N/A343S-FFZm exhibited the selectivity like LSZm but did not produce the beta-(2 -> 6)-fructoside-linked levan and/or long levanooligo-saccharides that LSZm did. We assumed Phe189(LSZm) to be a responsible residue for the elongation of levan chain in LSZm and mutated the corresponding Leu187(FFZm) in FFZm to Phe. An H79N/L187F/A343S-FFZm produced a higher quantity of long levanooligosaccharides than H79N/A343S-FFZm (or H79N-FFZm), although without levan formation, suggesting that LSZm has another structural factor for levan production. We also found that FFZm generated a sucrose analog, beta-D-fructofuranosyl alpha-D-mannopyranoside, by beta-fructosyltransfer to D-mannose and regarded His79(FFZm) and Ala343(FFZm) as key residues for this acceptor specificity. In summary, this study provides insight into the structural factors of regioselectivity and acceptor specificity in transfructosylation of GH68 enzymes.Elsevier2021-01engjournal articleNAhttp://hdl.handle.net/2115/82782https://doi.org/10.1016/j.jbc.2021.100398Journal of Biological Chemistry (JBC)296100398