2024-03-28T18:25:46Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/831942022-11-17T02:08:08Zhdl_2115_35410hdl_2115_35409Isolation and Characterization of Antimicrobial-Resistant Escherichia coli from Retail Meats from Roadside Butcheries in Uganda1000090762196Okubo, TorahikoYossapol, MontiraIkushima, ShioriKakooza, StevenWampande, Eddie M.1000010509764Asai, Tetsuo1000040734687Tsuchida, Sayaka1000050402219Ohya, Kenji1000030423122Maruyama, FumitoKabasa, John D.1000050183017Ushida, Kazunariopen accessFinal publication is available from Mary Ann Liebert, Inc., publishers https://doi.org/10.1089/fpd.2020.2796retail meatroadside butcheryUgandaE. coli490Retail meats are one of the main routes for spreading antimicrobial-resistant bacteria (ARB) from livestock to humans through the food chain. In African countries, retail meats are often sold at roadside butcheries without chilling or refrigeration. Retail meats in those butcheries are suspected to be contaminated by ARB, but it was not clear. In this study, we tested for the presence of antimicrobial-resistantEscherichia colifrom retail meats (n = 64) from roadside butcheries in Kampala, Uganda. The meat surfaces were swabbed and inoculated on PetriFilm SEC agar to isolateE. coli. We successfully isolatedE. colifrom 90.6% of these retail meat samples. We identified the phylogenetic type, antimicrobial susceptibility, and antimicrobial resistance genes prevalence between retail meat isolates (n = 89). Phylogenetic type B1 was identified from 70.8% of the retail meat isolates, suggesting that the isolates originated primarily from fecal contamination during meat processing. Tetracycline (TET)-resistant isolates withtetAand/ortetBgene(s) were the most frequently detected (28.1%), followed by ampicillin (AMP) resistance genes withbla(TEM)(15.7%,) and sulfamethoxazole-trimethoprim (SXT) resistance genes withsul2(15.7%). No extended-spectrum beta-lactamase-producing isolates were detected. A conjugation assay showed that resistance to AMP, TET, and SXT could be simultaneously transferred to recipients. These findings suggest that antimicrobial-resistantE. colican easily be transferred from farms to tables from retail meats obtained from roadside butcheries.Mary Ann Liebert2020-11-06engjournal articleAMhttp://hdl.handle.net/2115/83194https://doi.org/10.1089/fpd.2020.2796325519731535-31411556-7125Foodborne Pathogens and Disease1711666671https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/83194/1/Okubo_Resistant%20Ecoli%20from%20Retail%20Meat%20%28Roadside%29_Final.pdfapplication/pdf621.93 KB2020-11-06